Oxidative Stress Reagent via p-Phenylenediamine Derivative
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Solution Overview
Problem
Existing reagents for measuring oxidative stress, such as N,N-ethyl-p-phenylenediamine, have insufficient absorption characteristics in terms of sensitivity and wavelength range, limiting their effectiveness in accurately assessing oxidative stress levels.
Innovation Solution
A reagent comprising a compound represented by general formula (I) or its salt, which acts as a color-forming dye, is used to measure hydroperoxide levels in biological samples, offering improved sensitivity and a wider absorption wavelength range, allowing for more accurate oxidative stress assessment.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If N,N-ethyl-p-phenylenediamine is used as a color reagent for measuring oxidative stress, then the measurement can be performed, but the absorption characteristics are insufficient in terms of sensitivity and wavelength range
Solution Approach 1:
The patent applies parameter changes by modifying the chemical structure of the p-phenylenediamine derivative to optimize absorption characteristics. Specifically, it introduces compounds with different substituent groups (R1, R2, R3, R4) at specific positions on the phenylenediamine core structure, which systematically alter the absorption wavelength and sensitivity parameters to achieve superior measurement performance compared to conventional reagents like N,N-ethyl-p-phenylenediamine
2Adaptability or versatility
If N,N-ethyl-p-phenylenediamine is used as a color reagent, then the measurement can be performed, but the wavelength range is limited
Solution Approach 1:
The patent employs parameter changes by varying the substituent groups on the p-phenylenediamine core structure to achieve different absorption wavelengths. By systematically changing parameters such as the type of substituent (hydroxyl, alkoxy, halogen, etc.) and their positions, the reagent can be optimized for specific wavelength ranges while maintaining high absorption characteristics and measurement precision
Solution Approach 2:
The patent utilizes composite material principles by creating p-phenylenediamine derivatives with multiple functional groups that work synergistically. The core phenylenediamine structure provides the basic color-forming capability, while additional substituent groups enhance absorption characteristics and extend the usable wavelength range, creating a composite molecular structure with superior overall performance
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The reagent enables more sensitive and accurate measurement of oxidative stress across a broader wavelength range, reducing interference from serum components and variability in measurement devices, thereby enhancing the precision of oxidative stress evaluation.
Implementation Method 1
the concentration of the hydroperoxide is measured by the color reaction
Data Source
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AI summary
The present invention provides a reagent for measuring a degree of oxidative stress that includes a compound represented by the following general formula (I) or a salt thereof, and a method of measuring a degree of oxidative stress using the reagent for measuring a degree of oxidative stress. In the general formula (I), R1, R2, R3 and R4 each independently represent a hydrogen atom, an ethyl group, an isopropyl group, a hydroxyalkyl group that has 1 to 4 carbon atoms, or a phenyl group, and at least one of R1, R2, R3 or R4 represents an isopropyl group, a hydroxyalkyl group having 1 to 4 carbon atoms, or a phenyl group.