Oxygen-Controlled Seed Priming via Partial Pressure

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Solution Overview

Problem

Current seed priming techniques rely on water control, which faces challenges such as oxygen restriction, high solution volumes, disposal issues, and storability problems, and struggle to precisely stop the priming process, leading to reduced seedling emergence and storability.

Innovation Solution

The method involves controlling seed priming using partial oxygen pressure and carbon dioxide pressure instead of hydration levels, allowing metabolic activity for germination without radicle emergence, and then drying the seeds to maintain desiccation tolerance for storage.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Speed

If water control methods are used for seed priming, then germination speed is improved, but oxygen absorption is restricted and storability deteriorates

Engineering Contradiction:
Improvegermination speedVSAvoidoxygen restriction
Core Design Contradiction:
SpeedVSObject-affected harmful factors

Solution Approach 1:

The invention changes the control parameter from water potential to oxygen partial pressure. By regulating oxygen availability instead of water content, the method enables metabolic activation and germination speed improvement while avoiding the harmful effect of oxygen restriction that occurs in water-controlled priming methods.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The invention replaces the water-based mechanical hydration system with an oxygen-based gaseous control system. This substitution allows seeds to receive adequate oxygen for metabolism while still achieving controlled germination activation, thereby improving both germination speed and storability.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Manufacturing precision

If osmotic solutions are used for priming, then germination uniformity is improved, but solution volume and disposal problems increase

Engineering Contradiction:
Improvegermination uniformityVSAvoidsolution volume
Core Design Contradiction:
Manufacturing precisionVSQuantity of substance

Solution Approach 1:

The invention extracts and eliminates the need for large volumes of osmotic solutions by replacing them with a gaseous oxygen control system. This extraction removes the disposal problem associated with chemical solutions while maintaining the ability to achieve uniform germination through controlled oxygen partial pressure.

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The invention uses a controlled gaseous atmosphere with regulated oxygen partial pressure instead of liquid osmotic solutions. This inert environment approach provides precise control over seed metabolism and germination uniformity without requiring large quantities of chemical solutions that need disposal.

Inventive Principle:
Principle #39Inert atmosphere (Inert environment)

3Use of energy by moving object

If hydration level is increased for priming, then metabolic activity is enhanced, but desiccation tolerance is lost and storability decreases

Engineering Contradiction:
Improvemetabolic activityVSAvoidstorability
Core Design Contradiction:
Use of energy by moving objectVSReliability

Solution Approach 1:

The invention changes the controlling parameter from hydration level to oxygen partial pressure. This allows metabolic activity to be enhanced through controlled oxygen availability without increasing hydration to levels that would cause loss of desiccation tolerance, thereby maintaining storability.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The invention uses oxygen partial pressure as an intermediary control mechanism to stimulate metabolic activity. This intermediary approach enables germination activation without requiring high hydration levels, thus preserving the seed's desiccation tolerance and storability.

Inventive Principle:
Principle #24Intermediary (Mediator)

4Manufacturing precision

If water potential is controlled for priming, then germination synchronicity is improved, but precision in stopping the process deteriorates

Engineering Contradiction:
Improvegermination synchronicityVSAvoidprocess stopping precision
Core Design Contradiction:
Manufacturing precisionVSMeasurement precision

Solution Approach 1:

The invention changes the control parameter from water potential to oxygen partial pressure, which can be measured and regulated with higher precision using modern gas sensing and control technologies. This enables more precise stopping of the priming process while maintaining germination synchronicity.

Inventive Principle:
Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This approach enhances seed germination speed, synchronicity, and vigor while improving storability and reducing the risk of seed damage, leading to better crop yields and uniform seedling emergence.

Implementation Method 1

Respiration by mature 'dry' seeds (usual moisture content: 10-15%) of course is extremely low when compared with developing or germinating seeds

Methodology Applied
Scientific EffectRespiration: Aerobic Digestion

Data Source

PatentEP2154942B1Oxygen-controled priming of seed
Publication Date: 2020.02.12 FYTAGORAS BV
  • EP2154942B1 patent drawingFigure 1~2
  • EP2154942B1 patent drawingFigure 3~4
  • EP2154942B1 patent drawingFigure 5~6

AI summary

The present invention relates the methods, compositions and devices for priming of plant seed, preferably seed of crop plants, and seeds obtained with said methods, compositions and devices, wherein the metabolic activity necessary for germination of the seed is not controlled by water but rather by the partial oxygen pressure and/or partial carbon dioxide pressure. In the methods of the invention the partial oxygen or carbon dioxide pressure may be measured by means of an oxygen/carbon dioxide sensitive fluorescent dye. The invention further relates to processes for producing a primed seed lot of a plant species and to a container comprising primed seed under an atmosphere with a partial oxygen pressure that is below the critical oxygen pressure.