Oxygen Nanobubble Aqueous Solution for Cell Protection
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Solution Overview
Problem
Existing aqueous solutions containing oxygen nanobubbles for medical applications, such as physiological saline and infusion solutions, have inconsistent and insufficient effects in protecting cells under hypoxic or anaerobic conditions due to inadequate mean particle size and density of oxygen nanobubbles.
Innovation Solution
The development of an administrable aqueous solution with oxygen nanobubbles having a mean particle size of 30 nm or less and a density of 10^16 bubbles or more per ml, manufactured using a method that involves generating jets of aqueous solution through small holes to create oxygen nanobubbles with enhanced properties.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If oxygen nanobubbles with larger mean particle size (50 nm or more) are used in infusion solutions, then the solution can be manufactured with conventional methods, but the effect of protecting cells under hypoxic or anaerobic stimulation is insufficient and inconsistent
Solution Approach 1:
The patent changes the critical parameter of nanobubble mean particle size from 50 nm or more to 30 nm or less. This parameter change fundamentally improves cell protection effect consistency under hypoxic conditions while maintaining manufacturability through the jet generation method described in the patent
2Reliability
If oxygen nanobubbles with smaller mean particle size (30 nm or less) are used to improve cell protection effect, then the absorption and permeability are improved, but the manufacturing complexity increases
Solution Approach 1:
The patent employs pneumatic and hydraulic principles by generating oxygen nanobubbles through jet flow of aqueous solution through small holes. The jet flow mechanism creates nanobubbles of 30 nm or less in mean particle size, achieving improved cell protection effect while avoiding overly complex manufacturing equipment
Solution Approach 2:
The manufacturing method utilizes a structure with small holes (porous structure) through which the aqueous solution jets to generate nanobubbles. This approach simplifies the manufacturing process while consistently producing nanobubbles with the required small mean particle size for effective cell protection
3Quantity of substance
If the density of oxygen nanobubbles is increased to 10^16 bubbles/ml or more to enhance oxygen supply, then the oxygen content is sufficiently increased, but the stability of nanobubble suspension becomes challenging
Solution Approach 1:
The patent simultaneously optimizes multiple parameters: mean particle size reduced to 30 nm or less AND density increased to 10^16 bubbles/ml or more. This combined parameter optimization achieves sufficient oxygen content while the small particle size inherently improves suspension stability by reducing buoyancy effects
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This solution effectively protects cells from damage under hypoxic or anaerobic conditions and stably inhibits cancer cell proliferation by ensuring sufficient oxygen supply and improved absorption and permeability, reducing variations in effect over time.
Implementation Method 1
generating jets of aqueous solution through small holes to create oxygen nanobubbles
Implementation Method 2
amount of gas-dissolution is large
Data Source
Figure 1A~1B
Figure 2A~2B
Figure 3
AI summary
Provided are an administrable aqueous solution to a living body and a method for manufacturing the same, the administrable aqueous solution having a function of supplying sufficient oxygen to peripheral cells, causing less damage and injury of cells under a hypoxic or anaerobic stimulation, and continuously exhibiting a sufficiently high and stable effect to protect cells. The administrable aqueous solution to a living body according to the present invention contains oxygen nanobubbles having a mean particle size of 30 nm or less and a density of 1016 bubbles per ml, preferably a mean particle size of 1 to 10 nm and a density 1017 bubbles or more per ml, respectively, as determined by a measurement with a cryo-transmission electron microscope by an ice-embedding method. The administrable aqueous solution to a living body according to the present invention contains oxygen nanobubbles which are generated by jetting an aqueous solution containing dissolved oxygen from the outside of a cylinder, the cylinder being provided with 2 or more small through holes formed in the circumferential direction, via the small through-holes under atmospheric pressure or higher and thus bringing the jetted solution into collision in such a manner that water hammer is concentrated at the center of the cylinder on the same plane parallel to a cross-section in a radial direction of the cylinder.