Oyster Antioxidant Extraction via Solvent Segmentation

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Solution Overview

Problem

Current methods for producing antioxidants from oyster meat do not achieve a high content rate and degree of extraction of substances like taurine, glycogen, protein, and fat-soluble vitamins, and lack efficient extraction of antioxidative compounds such as 3,5-dihydroxy-4-methoxybenzyl alcohol, which are beneficial for health supplements.

Innovation Solution

A method involving storing raw oyster meat in an extraction solution, concentrating the extract, adding an ethanol solution, separating precipitate and supernatant, removing ethanol, concentrating and diluting the supernatant, and using ethyl acetate to recover the 3,5-dihydroxy-4-methoxybenzyl alcohol fraction, achieving a high extraction rate of antioxidative compounds.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If conventional extraction methods are used for oyster meat, then production is simple, but extraction efficiency and content rate of antioxidative compounds are low

Engineering Contradiction:
Improveextraction efficiencyVSAvoidprocess complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The extraction process is divided into multiple sequential steps: initial extraction, concentration, ethanol addition for precipitation, supernatant separation, and ethyl acetate extraction. Each step isolates and concentrates specific components, progressively improving the extraction efficiency of antioxidative compounds while managing process complexity through systematic segmentation

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

Concentration of the extract liquid is performed before adding ethanol solution and proceeding with further extraction steps. This preliminary concentration action increases the content rate of target compounds in the subsequent steps, improving overall extraction efficiency without requiring fundamentally new equipment or processes

Inventive Principle:
Principle #10Preliminary action

2Quantity of substance

If multiple extraction steps are added to improve extraction rate, then extraction efficiency increases, but manufacturing complexity increases

Engineering Contradiction:
Improvecontent rate of antioxidative compoundsVSAvoidmanufacturing ease
Core Design Contradiction:
Quantity of substanceVSEase of manufacture

Solution Approach 1:

The process utilizes systematic changes in solvent parameters (ethanol concentration, then ethyl acetate addition) to selectively extract different components. By changing solvent polarity and composition in controlled steps, the content rate of antioxidative compounds is maximized while using standard laboratory equipment and conventional extraction techniques

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

Specific antioxidative compounds are selectively extracted at different stages: initial extraction obtains general oyster components, then ethyl acetate extraction specifically isolates 3,5-dihydroxy-4-methoxybenzyl alcohol and other antioxidative compounds. This selective extraction approach increases compound content rate while using straightforward separation based on solubility differences

Inventive Principle:
Principle #2Taking out (Extraction)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This method results in a product with a high content of antioxidative properties, including 3,5-dihydroxy-4-methoxybenzyl alcohol, taurine, glycogen, and vitamin D, effectively enhancing the nutritional value and health benefits of oyster meat extracts for dietary supplements.

Implementation Method 1

storing raw oyster meat in an extraction container containing an extraction solution to obtain an extract liquid containing an oyster meat extract

Methodology Applied
Scientific EffectExtraction:

Implementation Method 2

extracting an active ingredient extract from oyster meat

Methodology Applied
Scientific EffectDiffusion: Diffusion

Implementation Method 3

concentrating the extract liquid

Methodology Applied
Scientific EffectConcentration:

Implementation Method 4

adding an ethanol solution to the concentrated extract liquid obtained in step (2) to achieve a solution with an ethanol concentration of approximately 70%; stirring the resulting liquid from step (3) thereby separating precipitate from supernatant

Methodology Applied
Scientific EffectPrecipitation: Precipitation

Implementation Method 5

adding ethyl acetate to the liquid obtained in step (7) to achieve a solution; separating the solution obtained in step (8) into an ethyl acetate layer and a water layer; recovering a 3,5-dihydroxy-4-methoxybenzyl alcohol fraction from the ethyl acetate layer

Methodology Applied
Scientific EffectLiquid-liquid extraction: Liquid-Liquid Extraction

Data Source

PatentEP2669353B1Antioxidant, antioxidant composition and production method therefor
Publication Date: 2020.11.04 WATANABE OYSTER LAB
  • EP2669353B1 patent drawingFigure 1
  • EP2669353B1 patent drawingFigure 2
  • EP2669353B1 patent drawingFigure 3

AI summary

Problem To provide an antioxidant, an antioxidant composition, and a method for producing the antioxidant and the antioxidant composition, which feature a high content rate and degree of extraction of substances. These substances are taurine, glycogen, protein, so-called blood platelet anticoagulant with zinc, fat-soluble vitamin with a high activation such as vitamin D, and other useful substances. The antioxidant and the antioxidant composition also feature a so-called antioxidative property, which has recently attracted attention. Solution The present invention, for example, includes 3,5-dihydroxy-4-methoxybenzyl alcohol.