Oyster Peptide Enzymolysis And Purification for Targeted Functional Segments
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Solution Overview
Problem
Existing oyster peptide production methods lose functional peptides during enzymolysis, narrowing their application scope and hindering the development of oysters as a deep-processing product.
Innovation Solution
A controlled enzymolysis and purification process is employed to produce oyster peptides with specific peptide segments (Ile-Arg, Arg-Ile, and Val-Arg) at targeted mass contents, using a combination of neutral protease and papain, followed by cation and hydrophobic chromatography, to enhance absorption and efficacy.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If conventional enzymolysis is used to produce oyster peptides for general health benefits, then nutritional absorption is improved, but functional peptides with specific activities (such as sexual function enhancement) are lost
Solution Approach 1:
The patent applies parameter changes by precisely controlling enzymolysis conditions (enzyme type, concentration, temperature, time, pH) to selectively preserve specific functional peptide segments (Ile-Arg, Arg-Ile, Val-Arg) while maintaining overall peptide quality. This targeted parameter optimization enables the production of peptides with specific biological activities for sexual function enhancement while preserving general health benefits.
Solution Approach 2:
The patent extracts and isolates specific functional peptide segments from the complex mixture of oyster protein hydrolysis products. Through chromatography and filtration processes, the functional peptides (Ile-Arg, Arg-Ile, Val-Arg) are separated and concentrated to achieve minimum mass content requirements, thereby preserving their specific biological activities for sexual function enhancement.
2Speed
If enzymolysis is performed to break down oyster proteins into small peptides, then absorption rate is improved, but peptide diversity and functional specificity are reduced
Solution Approach 1:
The patent optimizes enzymolysis parameters including enzyme selection (neutral protease, papain), concentration, temperature (37-45°C), time (2-6 hours), and pH (7-9) to achieve precise control over peptide composition. These parameter changes ensure both rapid absorption (small peptide size) and high precision in functional peptide content (Ile-Arg ≥7.60 mg/100g, Arg-Ile ≥3.60 mg/100g, Val-Arg ≥6.50 mg/100g).
Solution Approach 2:
The patent implements feedback control by monitoring peptide composition during enzymolysis and adjusting process parameters accordingly. Mass spectrometry and other analytical methods are used to detect functional peptide segments, allowing real-time optimization of enzymolysis conditions to maintain target mass content levels while ensuring rapid absorption characteristics.
3Quantity of substance
If oysters are processed into peptides for general health purposes, then nutritional value is improved, but specific physiological effects (such as testosterone promotion) are lost
Solution Approach 1:
The patent extracts and isolates specific functional peptide segments (Ile-Arg, Arg-Ile, Val-Arg) that are responsible for promoting testosterone secretion and improving sexual function. Through chromatography and filtration, these biologically active peptides are separated from other hydrolysis products, ensuring both nutritional value and specific physiological effects are preserved.
Solution Approach 2:
The patent optimizes processing parameters to preserve the biological activity of functional peptides while maintaining nutritional content. By controlling enzymolysis conditions, pH, temperature, and enzyme-to-substrate ratios, the patent ensures that both the quantity of nutritional peptides and their specific physiological effects (testosterone promotion, sexual function enhancement) are maintained at required levels.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The resulting oyster peptides effectively promote testosterone and dihydrotestosterone secretion, improving sexual function and broadening their application scope beyond conventional health benefits.
Implementation Method 1
enzymolysis treatment (with a neutral protease and a papain)
Implementation Method 2
a protein denaturation treatment (an alkali treatment), an enzymolysis treatment (with a neutral protease and a papain)
Implementation Method 3
a separation and purification treatment; where, in the enzymolysis treatment, an enzymolysis time is 3-6 h
Implementation Method 4
followed by cation and hydrophobic chromatography
Data Source
AI summary
An oyster peptide with an effect of improving sexual function and a preparation method thereof are provided, the oyster peptide at least includes peptide segments RI, IR and VR in its composition. Based on a mass of the oyster peptide, a content of the RI is ≥3.60 mg/100 g, a content of the IR is ≥7.60 mg/100 g, and a content of the VR is ≥6.50 mg/100 g.