Oyster Shell Fraction Isolation via Citric Acid Reaction
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Solution Overview
Problem
Current methods for treating inflammatory responses and osteoarthritis are limited by the side effects of topical steroids and non-steroidal anti-inflammatory drugs, and there is a need for more effective anti-inflammatory agents that can reduce energy costs and simplify the isolation of fractions with anti-inflammatory or osteoarthritis-inhibiting effects.
Innovation Solution
A method involving the reaction of oyster shell with anhydrous citric acid at a relatively low temperature, followed by centrifugation and ultra-filtration to isolate a fraction with anti-inflammatory or osteoarthritis-inhibiting effects, reducing energy costs and simplifying the process.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional methods are used to treat inflammatory responses and osteoarthritis, then therapeutic effects can be achieved, but side effects occur due to topical steroids and non-steroidal anti-inflammatory drugs
Solution Approach 1:
The patent extracts and utilizes specific fractions from oyster shell that possess anti-inflammatory and osteoarthritis-inhibiting effects. By isolating the active components (fractions with molecular weights of 3-10 kDa and 10-30 kDa) from the oyster shell matrix through enzymatic hydrolysis and ultrafiltration, the invention obtains therapeutic agents that exert anti-inflammatory effects without the harmful side effects associated with conventional drugs like topical steroids and non-steroidal anti-inflammatory drugs.
2Productivity
If high energy input methods are used for processing oyster shell, then reaction efficiency can be improved, but energy costs increase
Solution Approach 1:
The patent utilizes enzymatic hydrolysis with proteinase K at relatively low temperatures (37-50°C) to convert oyster shell proteins into soluble peptides and amino acids. This biochemical approach replaces high-energy physical or chemical methods, achieving efficient decomposition of oyster shell components while maintaining low energy consumption. The enzymatic reaction conditions (pH 7.5-8.5, 37-50°C) are optimized to maximize reaction efficiency without requiring high energy input.
3Manufacturing precision
If complex isolation procedures are used to obtain anti-inflammatory fractions, then purity can be improved, but process complexity increases
Solution Approach 1:
The patent employs ultrafiltration membranes with specific molecular weight cutoffs (3 kDa, 10 kDa, 30 kDa) to segment and separate the hydrolyzed oyster shell components into distinct fraction ranges. This segmentation approach efficiently isolates the anti-inflammatory active components (3-10 kDa and 10-30 kDa fractions) from other components through a systematic multi-stage filtration process, achieving high purity without requiring complex chromatographic or centrifugation procedures.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method effectively isolates a fraction with significant anti-inflammatory and osteoarthritis-inhibiting effects, reducing energy costs and providing a simpler process, as demonstrated by the high COX-2 inhibitory activity and reduced inflammatory cytokine production.
Implementation Method 1
reacting the powder with anhydrous citric acid to obtain a reaction product
Implementation Method 2
centrifuging the reaction product to obtain a supernatant
Implementation Method 3
subjecting to ultra-filtration
Data Source
AI summary
The present invention relates to a method for isolating a fraction having anti-inflammatory or osteoarthritis-inhibiting effects by removing impurities and washing oyster shells, which are produced during a process of producing oysters, drying same, forming a powder from the oyster shells, and adding anhydrous citric acid to same for a reaction, obtaining a reaction product, and isolating a supernatant, which is obtained by centrifugally separating the reaction product, by means of ultrafiltration. By adding the anhydrous citric acid to the oyster shell chips for the reaction at a relatively low temperature, the present invention provides superior advantages, such as significantly reducing energy costs and isolating the fraction having the anti-inflammatory or osteoarthritis inhibiting effects through a simple method.