Oxygen-Ozone Blood Pre-treatment for Stem Cell Viability

Solution Overview

Problem

Current methods for separating stem cells from blood often cause cell damage due to centrifugation, leading to reduced effectiveness when transplanted.

Innovation Solution

Pre-treating blood with an oxygen-ozone mixture before separation, using a specific ratio and concentration of oxygen and ozone, enhances stem cell quality, viability, and quantity, allowing for effective separation without centrifugation or with minimal damage.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If centrifugation is used to separate stem cells from blood, then stem cells can be separated and retrieved, but the cells suffer stress and damage reducing their effectiveness

Engineering Contradiction:

Improvestem cell separation efficiencyVSAvoidcell damage and stress

Core Design Contradiction:

ProductivityVSObject-affected harmful factors

Solution Approach 1:

The patent applies preliminary action by pre-treating the blood with oxygen-ozone mixture before the separation process. This pre-treatment enhances stem cell quality and viability in advance, making the cells more resilient to subsequent separation procedures and reducing damage during the process.

Inventive Principle:

Principle #10Preliminary action

Solution Approach 2:

The patent changes the chemical composition parameters of the blood by introducing oxygen-ozone mixture with specific concentrations (ozone 5-50 μg/ml, oxygen 20-95% v/v). This parameter change activates and protects stem cells, improving their survival rate and effectiveness while maintaining separation efficiency.

Inventive Principle:

Principle #35Parameter changes

2Device complexity

If conventional separation techniques are used without pre-treatment, then the separation process is simpler, but stem cell quality and viability are reduced

Engineering Contradiction:

Improveseparation process complexityVSAvoidstem cell viability and quality

Core Design Contradiction:

Device complexityVSReliability

Solution Approach 1:

The patent applies preliminary action by pre-treating the blood with oxygen-ozone mixture before the separation process. This pre-treatment enhances stem cell quality and viability in advance, making the cells more resilient to subsequent separation procedures and reducing damage during the process.

Inventive Principle:

Principle #10Preliminary action

Solution Approach 2:

The patent changes the chemical composition parameters of the blood by introducing oxygen-ozone mixture with specific concentrations (ozone 5-50 μg/ml, oxygen 20-95% v/v). This parameter change activates and protects stem cells, improving their survival rate and effectiveness while maintaining separation efficiency.

Inventive Principle:

Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The pre-treatment with oxygen-ozone mixture results in more numerous, viable, and active stem cells, improving their effectiveness when transplanted and maintaining quality through cryopreservation.

Implementation Method 1

pre-treating blood cells from patient's blood using an oxygen-ozone mixture

Methodology Applied

Scientific EffectOxidation: Oxidation

Data Source

PatentUS9988601B2Method for retrieval of stem cells from human and animal blood using an oxygen-ozone mixture

Publication Date: 2018.06.05 PAOLO GOBBI FRATTINI

AI summary

The present invention relates to a method for retrieving more numerous, viable and efficient stem cells from the blood of a patient. The method comprises a preliminary step of pre-treating blood cells using an oxygen-ozone mixture and a subsequent step of separating the stem cells from said pre-treated blood cells.