PAPS Amine Salt Stability via Lyophilization
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Solution Overview
Problem
Solid PAPS metal salts, such as lithium and sodium salts, are highly unstable at ambient temperature, making it difficult to isolate and store highly pure PAPS in a stable state, hindering industrial applications.
Innovation Solution
A stable salt of PAPS is formed by combining PAPS with a secondary, tertiary, or polyamine, or the amino acid L-histidine, followed by lyophilization, which significantly enhances stability.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Stability of the object's composition
If PAPS is synthesized as a metal salt (lithium or sodium salt) in solid form, then it can be isolated and stored, but it decomposes rapidly at ambient temperature
Solution Approach 1:
The patent changes the cation parameter from metal ions (Li+, Na+) to organic ammonium ions with specific structures. This parameter change transforms the chemical properties of PAPS salt, resulting in enhanced stability at ambient temperature while maintaining the desired solid form for isolation and storage.
Solution Approach 2:
The patent creates composite salt structures by combining PAPS anion with specifically designed organic ammonium cations containing hydrophobic groups. This composite approach combines the stability of organic structures with the functional properties of PAPS, resolving the contradiction between isolability and storage stability.
2Ease of manufacture
If conventional synthesis methods are used to produce PAPS metal salt powder, then production is straightforward, but the product is highly unstable and difficult to maintain in pure form
Solution Approach 1:
The patent modifies the synthesis parameters by changing the cation selection from metals to organic ammonium ions. This parameter change enables straightforward production through conventional methods while simultaneously achieving high purity and stability, as the organic salts resist decomposition and maintain purity during storage.
3Ease of operation
If PAPS is stored in solid form at ambient temperature, then storage is convenient, but decomposition occurs rapidly
Solution Approach 1:
The patent changes the chemical composition parameter from metal salts to organic ammonium salts, which fundamentally alters the thermal and chemical stability profile. This enables convenient ambient temperature storage while extending the storage duration significantly, as the organic salts do not decompose rapidly like their metal counterparts.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The resulting amine salt of PAPS exhibits improved stability, maintaining 80% or more undecomposed PAPS after seven days at ambient temperature, allowing for stable storage and use without decomposition concerns.
Implementation Method 1
a salt prepared from PAPS and an amine compound... has excellent stability
Implementation Method 2
lyophilizing the resultant solution
Data Source
AI summary
The present invention provides a stable salt of 3'-phosphoadenosine 5'-phosphosulfate (PAPS) and a production method therefor. The present invention is directed to a stable salt of PAPS (amine salt), which is formed between PAPS and an amine compound, and to a method for producing a stable salt of PAPS, which includes adding an amine compound to an aqueous PAPS solution in an amount by mole equal to or greater than that of PAPS, and lyophilizing the resultant solution. The present invention has first realized production of a solid-form PAPS salt having considerably improved stability through a very simple technique. Since the thus-produced amine salt of PAPS is very stable, the salt can be stored or employed without taking much care about decomposition thereof at ambient temperature.