Parallel Base Sequencing Control With Split Reaction Components
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Solution Overview
Problem
Current sequencing technologies are inefficient in terms of sequencing time and cost, particularly due to the sequential nature of biochemical reactions and data collection processes.
Innovation Solution
The method involves dividing the reaction device into at least two components, where one component undergoes a biochemical reaction while the other is photographed, utilizing a fluid device and an optical device in parallel to perform biochemical reactions and data collection simultaneously, thereby optimizing the sequencing process.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If sequential biochemical reactions and data collection are performed one after another, then the process is simple to control, but the sequencing time is long and efficiency is low
Solution Approach 1:
The reaction device is divided into multiple independent reaction components (first component, second component, etc.), allowing different components to perform biochemical reactions and data collection operations in parallel. This segmentation enables simultaneous processing of multiple samples or multiple steps of the same sample, thereby reducing total sequencing time and improving efficiency.
Solution Approach 2:
The patent introduces a spatial dimension by using multiple reaction components arranged in parallel, transforming the traditional sequential one-dimensional process into a multi-dimensional parallel processing system. This allows biochemical reactions and optical detection to occur simultaneously across different components, effectively reducing the time loss associated with sequential operations.
2Productivity
If a single component performs all biochemical reactions and data collection, then the device structure is simple, but the sequencing cost increases due to repeated operations
Solution Approach 1:
The reaction device is segmented into multiple independent components, each capable of performing biochemical reactions and data collection. This segmentation reduces the need for repeated operations on a single component, thereby reducing overall sequencing cost while maintaining manageable device complexity through modular design.
Solution Approach 2:
Each reaction component is designed with multi-functionality, capable of performing both biochemical reactions and data collection operations. This universality reduces the need for separate dedicated components for each function, balancing device complexity with improved productivity and reduced sequencing costs.
3Loss of time
If biochemical reactions and photographing are performed sequentially on the same component, then the operation is straightforward, but the sequencing time is extended
Solution Approach 1:
The operation process is segmented across multiple components, allowing biochemical reactions to be performed on one component while data collection is performed on another component simultaneously. This segmentation reduces total reaction and detection time while the control system manages the increased complexity through automated coordination of multiple components.
Solution Approach 2:
The patent implements continuous useful action by overlapping biochemical reactions and data collection operations in time and space. While one component is performing a biochemical reaction, another component is simultaneously performing data collection, ensuring that no time is wasted for either process and maintaining continuous productive activity throughout the sequencing workflow.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach reduces sequencing time and improves efficiency by effectively utilizing both optical and fluid devices, while also reducing overall sequencing costs.
Implementation Method 1
The detection module generally includes an optical detection module, a current detection module and a acid-base (pH) detection module. The sequencing platform based on the optical detection principle is used for sequence determination by analyzing variation in the optical signals collected from a sequencing biochemical reaction.
Data Source
AI summary
The present disclosure relates to base sequence determination. An example system includes a fluid device and an optical device, a reaction device includes a first component and a second component, and a repeated executable unit included in the base sequence determination defined as: a second biochemical reaction—a first biochemical reaction—photographing. An example method includes, after initiation steps, using the fluid device to perform the second biochemical reaction and the first biochemical reaction of the sample on the first component, while using the optical device to photograph the sample on the second component. The initial steps include: using the fluid device to perform the first biochemical reaction of the sample on the first component, using the optical device to photograph the sample on the first component after the first biochemical reaction, and using the fluid device to perform the first biochemical reaction of the sample on the second component.