Parallel Bioreactor System Using Oscillating Motion for Cell Culture
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Solution Overview
Problem
Conventional bioreactors face challenges in achieving high throughput screening and optimizing cell culture processes due to high shearing forces and inadequate control over microenvironment parameters, which limits the production of biological drugs, especially for sensitive cells and microorganisms.
Innovation Solution
A parallel bioreactor system featuring oscillating culture vessels with inverted truncated conical inner cavities and a control system for precise parameter control, minimizing shearing forces and optimizing dissolved oxygen levels, allowing for simultaneous culture of multiple samples under controlled conditions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Use of energy by moving object
If traditional stirring or bubbling methods are used for oxygen transfer and mixing in bioreactors, then oxygen transfer efficiency is improved, but shearing force increases causing damage to animal cells
Solution Approach 1:
The patent applies oscillating motion to the culture vessel to achieve liquid circulation and oxygen transfer without traditional stirring blades or bubbling. The oscillation creates gentle fluid movement that maintains cell viability while ensuring adequate oxygen supply and mixing of culture media.
Solution Approach 2:
The patent replaces the traditional mechanical stirring system with an oscillating motion system. Instead of using rotating impellers or blades that create high shearing forces, the system uses controlled oscillation of the entire culture vessel to achieve mixing and oxygen transfer through gentle fluid dynamics.
2Productivity
If conventional bioreactors are used for cell culture, then basic culture functions are achieved, but high throughput screening and parallel culture capability are insufficient
Solution Approach 1:
The patent divides the bioreactor system into multiple independent culture vessels that can operate in parallel. Each vessel functions as an independent culture unit, enabling simultaneous screening of multiple cell clones or culture conditions while maintaining individual control over each vessel's parameters.
Solution Approach 2:
The patent designs a universal culture vessel structure with standardized interfaces and control systems that can accommodate different cell types and culture conditions. The same basic vessel design serves multiple functions across different screening experiments, reducing overall system complexity while enabling high throughput capability.
3Adaptability or versatility
If conventional bioreactors are used, then basic culture operations are possible, but independent control of microenvironment parameters in each culture vessel is limited
Solution Approach 1:
The patent provides each culture vessel with independent control systems for parameters such as temperature, pH, and oxygen levels. This segmentation of control functions allows each vessel to maintain its own optimal microenvironment independently, enabling precise adaptation to different cell culture requirements without requiring complex centralized control.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The system enables high throughput screening and efficient culture process optimization by reducing shearing forces, improving cell growth density, and allowing independent control of each culture vessel, thereby enhancing the production of biological drugs.
Implementation Method 1
an oscillator for generating oscillating motion; a plurality of culture vessels mounted on the oscillator
Implementation Method 2
each culture vessel is provided with an inner cavity, the inner cavity comprises a cylindrical portion at the upper part and an inverted truncated conical bottom at the lower part
Data Source
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AI summary
According to the invention, there is provided a parallel bioreactor system, comprising: an oscillator for generating oscillating motion; a plurality of culture vessels mounted on the oscillator, wherein each culture vessel is provided with an inner cavity, the inner cavity comprises a cylindrical portion at the upper part and an inverted truncated conical bottom at the lower part, a cross section of the cylindrical portion is consistent with the cross section of the top of the inverted truncated conical bottom, and the bottom of the cylindrical portion is joined with the top of the inverted truncated conical bottom; disposable culture bags arranged in the inner cavities of the culture vessels and used for accommodating culture solution, wherein each disposable culture bag is provided with a multifunctional cover plate, and the multifunctional cover plate is connected to the top of the culture bag to seal the culture bag, and is provided with a plurality of connection holes leading to interior of the disposable culture bag; and a control system, wherein the control system controls the oscillating motion of the oscillator and parameters of the culture solution in the disposable culture bags.