Particle Analyzer Staining Abnormality Detection via Dual Control Particles
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Solution Overview
Problem
Existing particle analyzers cannot discern abnormalities in the staining step, leading to inaccurate measurement results due to the inability to differentiate between detection and staining issues.
Innovation Solution
A method involving the use of first control particles that are stained with a fluorescent dye and second control particles that emit fluorescence but are not stained, where the ratio of their detected fluorescence values is used to determine abnormalities in the staining step, allowing for separate analysis of detection and staining performance.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If quality control is performed using only first standard particles and second standard particles with fixed reference ranges, then abnormality in fluorescence detector or specimen preparation mechanism can be determined, but abnormality in the staining step cannot be discerned
Solution Approach 1:
The invention segments the quality control process into three distinct evaluation dimensions: (1) fluorescence detector performance using second standard particles, (2) specimen preparation mechanism using first standard particles, and (3) staining step performance using the ratio between first and second standard particles. This segmentation allows independent evaluation of each component, enabling identification of staining abnormalities that were previously indistinguishable from other sources of error.
2Device complexity
If the particle analyzer uses a single quality control substance with fixed reference ranges, then the device complexity is low, but the measurement precision for identifying specific abnormality sources is insufficient
Solution Approach 1:
The invention introduces a ratio value as an intermediary parameter that mediates between the fluorescence intensity measurements of first and second standard particles. This ratio serves as a specific indicator for staining step performance, allowing the system to precisely identify staining abnormalities without requiring complex multi-step procedures. The intermediary ratio simplifies the evaluation while maintaining high precision.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables accurate identification of staining abnormalities, preventing inaccurate analysis and ensuring proper functioning of the particle analyzer by distinguishing between detection and staining issues, thereby improving measurement reliability.
Implementation Method 1
first control particles which are to be stained by the fluorescent dye
Implementation Method 2
second control particles which are not to be stained by the fluorescent dye and which emit fluorescence
Data Source
Figure 1
Figure 2A~2D
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AI summary
Disclosed is a method for determining abnormality in a particle analyzer (10). The method includes: staining first control particles but not staining second control particles which emit fluorescence; irradiating with light the first control particles and the second control particles flowing in a flow cell, and detecting fluorescence from the first control particles and the second control particles; obtaining a first management value indicating a detection result of the fluorescence emitted from the first control particles and a second management value indicating a detection result of the fluorescence emitted from the second control particles; and determining abnormality in the staining step, based on a value calculated from the first management value and the second management value or a ratio between the first management value and the second management value.