Particle Trapping Chip Barcode Correlation for Single Cell Analysis
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Solution Overview
Problem
Current methods for confirming the movement and position of particles in single cell analysis are labor-intensive and time-consuming, especially when dealing with multiple cells, as they require manual tracing of cells using a camera.
Innovation Solution
A particle confirming method that involves correlating identification information with position information of particles trapped in wells, discharging the particles, acquiring identification information post-discharge, and confirming the particle's original position based on the acquired information.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If manual tracing of cells using a camera is performed to confirm particle movement and position, then particle position confirmation is achieved, but time consumption and labor intensity increase significantly
Solution Approach 1:
The patent applies preliminary action by attaching identification information (such as barcodes or fluorescent labels) to particles before discharge. This allows the particle analysis system to automatically identify and track particles after they are discharged from microwells, eliminating the need for manual tracing during the confirmation process. The identification information is prepared in advance, enabling automated detection and position confirmation.
Solution Approach 2:
The patent replaces the mechanical manual tracing operation with an automated optical detection system. Instead of manually moving a camera to trace particles, the system uses optical fields (fluorescence detection, barcode scanning) to automatically detect particle positions and identification information. This substitution of mechanical operation with optical/electronic detection dramatically reduces time consumption while maintaining measurement precision.
2Measurement precision
If manual tracing of cells using a camera is performed to confirm particle movement and position, then particle position confirmation is achieved, but labor intensity increases significantly
Solution Approach 1:
The patent applies self-service by enabling particles to carry their own identification information that automatically identifies them during detection. The particle analysis system automatically reads the identification information (barcodes, fluorescent labels) attached to particles, and automatically correlates this with position information from detection images. This eliminates the need for manual tracing operations, significantly reducing labor intensity while maintaining measurement precision.
Solution Approach 2:
The patent replaces manual mechanical tracing with automated optical detection and image processing systems. The system automatically captures images, detects particle positions, reads identification information, and correlates data without human intervention. This automation transforms a labor-intensive manual operation into an automated process, dramatically improving ease of operation while preserving measurement accuracy.
3Reliability
If camera visual field movement is performed to track particles during fractionation, then complete particle tracking is achieved, but operation complexity and time consumption increase
Solution Approach 1:
The patent applies preliminary action by pre-attaching identification information to particles before fractionation. This allows the particle analysis system to automatically identify particles at their new positions after discharge without needing to continuously track their movement. The identification information serves as a permanent marker that simplifies the detection process, maintaining tracking completeness while reducing operational complexity.
Solution Approach 2:
The patent replaces the complex mechanical operation of moving camera visual fields with automated optical detection. The system uses fluorescence detection or barcode scanning to automatically identify particles at any position within the detection field, eliminating the need for manual visual field movement. This substitution maintains reliable particle tracking while dramatically reducing operational complexity and making the process suitable for high-throughput fractionation of multiple particles.
Data Source
AI summary
To provide a technology for confirming that a desired particle is recovered in single cell analysis.The present technology provides a particle confirming method including a correlating step of correlating ID information possessed by a particle trapped in a well in a particle trapping region with position information of the well, a discharging step of discharging the particle from the well, an ID information acquiring step of acquiring ID information of the particle after the discharging step, and a confirming step of confirming the position of the well in which the particle has been trapped, on the basis of the acquired ID information. In addition, the present technology also provides a particle trapping chip and a particle analyzing system to be used for carrying out the method.