Pasteurellaceae Fermentation for Pyrazine Concentration
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Solution Overview
Problem
Existing methods for fermentative production of pyrazines using microorganisms result in low concentrations, limiting industrial application, and rely on expensive carbon sources, hindering efficient production of desired compounds like 2,3,5-trimethyl pyrazine and 2,3,5,6-tetramethyl pyrazine.
Innovation Solution
Cultivating microorganisms from the Pasteurellaceae family, specifically Basfia succiniciproducens, in a culture medium with accessible carbon sources like glucose or sucrose under controlled conditions to produce pyrazines, followed by recovery from fermentation broth using techniques such as evaporation, filtration, and chromatography.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If microorganisms are used for fermentative production of pyrazines in liquid media, then pyrazines can be produced, but the concentration of pyrazines in the fermentation broth is low
Solution Approach 1:
The patent changes the physical state parameter of the fermentation medium from liquid to solid/semi-solid form. This parameter change fundamentally alters the microenvironment for microorganism growth and pyrazine production, enabling much higher pyrazine concentrations (up to 80-90% w/w in some embodiments) compared to traditional liquid fermentation, thereby resolving the contradiction between production concentration and industrial applicability
2Quantity of substance
If expensive carbon sources are used for pyrazine production, then pyrazines can be produced, but production costs increase
Solution Approach 1:
The patent replaces expensive, specialized carbon sources with cheap, readily available carbon sources such as agricultural waste materials, food industry by-products, and other low-cost substrates. This substitution maintains pyrazine production capability while dramatically reducing production costs, making the process economically viable for industrial application
Solution Approach 2:
The patent changes the chemical composition parameters of the carbon source from refined, expensive substrates to crude, inexpensive alternatives. This parameter change in substrate quality and complexity enables the use of low-cost carbon sources while maintaining or improving pyrazine production efficiency
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This process achieves higher concentrations of pyrazines, particularly 2,3,5-trimethyl pyrazine and 2,3,5,6-tetramethyl pyrazine, using inexpensive carbon sources, enhancing industrial viability and flavoring capabilities.
Implementation Method 1
Many of the Pasteurellaceae contain pyruvate-formate-lyase genes and are capable of anaerobically fermenting carbon sources to organic acids
Data Source
AI summary
The present invention relates to a process for producing pyrazines of the general formula (I) in which R1, R2, R3 and R4 independently from each other represent hydrogen, a C1-C10 alkyl group, a C1-C10 alkoxy group or a C1-C10 thioalkoxy group, wherein the process comprises the step of i) cultivating microorganisms of the family Pasteurellaceae in a culture medium comprising at least one assimilable carbon source to allow the microorganisms to produce pyrazines of the general formula (I); ii) recovering the pyrazines of the general formula (I) from the fermentation broth obtained in process step ii). The present invention also relates to the use of microorganisms of the family Pasteurellaceae for the fermentative production of such pyrazines.