Patterned Nucleotide Chip Depressions for Stable Sequencing
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Solution Overview
Problem
Existing nucleic acid sequencing chips face challenges in achieving stable and accurate sequencing results due to the influence of geometric structure shape and dimensions on the chip surface, affecting parameters such as clonal cluster stability, signal intensity, and sequencing accuracy.
Innovation Solution
A patterned chip design with regularly arranged depressions featuring specific geometric structures, including interconnected first and second geometric structures with defined dimensions and angles, enhances sequencing stability and accuracy by improving detection throughput and surface utilization.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Area of stationary object
If geometric structures with specific shapes are formed on the chip surface, then the arrangement density and surface utilization rate are improved, but the sequencing stability and accuracy are affected
Solution Approach 1:
The patent applies parameter changes by precisely controlling the geometric parameters of the depression structures, including depth (0-100 μm), maximum dimension (10 nm-100 μm), and side wall angle (10-170°). By optimizing these parameters, the chip achieves both high surface utilization rate and stable sequencing results, resolving the contradiction between area utilization and sequencing reliability.
2Area of stationary object
If geometric structures with specific shapes are formed on the chip surface, then the arrangement density is improved, but the sequencing accuracy is affected
Solution Approach 1:
The patent uses parameter changes to optimize the depression geometry, specifying depth (0-100 μm), maximum dimension (10 nm-100 μm), and side wall angle (10-170°). These controlled parameter variations enable high arrangement density while maintaining sequencing accuracy through physical confinement of nucleic acid molecules.
Solution Approach 2:
The patent applies local quality by creating depressions with specific geometric characteristics at different locations on the chip surface. The interconnected first and second geometric structures provide localized confinement zones that optimize both arrangement density and sequencing accuracy for nucleic acid molecules at each site.
3Productivity
If geometric structures with specific shapes are formed on the chip surface, then the detection throughput is improved, but the signal intensity and quality parameters are affected
Solution Approach 1:
The patent applies parameter changes by optimizing the depression depth (0-100 μm) and dimensions to create optimal confinement zones that enhance signal intensity while maintaining high detection throughput. The geometric parameters are tuned to balance productivity and measurement precision.
Data Source
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AI summary
The present application provides a chip, which includes a chip substrate, wherein the chip substrate is provided with regularly arranged depressions on at least one surface; the depression comprises, in sequence, a first geometric structure and a second geometric structure that are interconnected in a direction from a surface of the chip substrate toward a central part of the chip substrate. In the chip provided by the examples of the present application, the regularly arranged depressions are formed on the substrate, and the depression comprises, in sequence, the first geometric structure and the second geometric structure that are interconnected in the direction from the surface of the chip substrate toward the central part of the chip substrate, so that the morphological structure of the depressions in the present application facilitates substance exchange and diffusion between the liquid phase of a reaction reagent and active molecules on the surface of the chip during a biochemical reaction in a detection process (e.g., nucleic acid sequencing), thereby promoting the reaction to occur and thus obtaining better detection signals (including signal intensity and signal-to-noise ratio) and more accurate detection results.