PCR Primer Sets for IMP Carbapenemase Detection
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Solution Overview
Problem
Current methods for detecting IMP carbapenemases are inadequate due to the genetic variability of the IMP family of β-lactamases, leading to insufficient detection of all IMP genes associated with gram-negative infections, which hampers antibiotic stewardship and infection control.
Innovation Solution
The use of specific sets of PCR primers associated with probes for real-time PCR assays to identify specific families of IMP β-lactamases, and the combination of multiple primer sets with fluorescent dyes to detect a broad range of IMP variants, facilitating their classification and detection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional molecular techniques are used to detect IMP carbapenemases, then the detection method is simple, but the detection accuracy is insufficient due to genetic variability of IMP family
Solution Approach 1:
The patent segments the detection approach by dividing IMP variants into specific families (IMP-1, IMP-4, IMP-7, IMP-8, IMP-9, IMP-10, IMP-12, IMP-14, IMP-16, IMP-18, IMP-19, IMP-20, IMP-21, IMP-22, IMP-23, IMP-24, IMP-25, IMP-26, IMP-27, IMP-28, IMP-29, IMP-30, IMP-31, IMP-32, IMP-33, IMP-34, IMP-35, IMP-36, IMP-37, IMP-38, IMP-39, IMP-40, IMP-41, IMP-42, IMP-43, IMP-44, IMP-45, IMP-46, IMP-47, IMP-48, IMP-49, IMP-50, IMP-51, IMP-52, IMP-53, IMP-54, IMP-55, IMP-56, IMP-57, IMP-58, IMP-59, IMP-60, IMP-61, IMP-62, IMP-63, IMP-64, IMP-65, IMP-66, IMP-67, IMP-68, IMP-69, IMP-70, IMP-71, IMP-72, IMP-73, IMP-74, IMP-75, IMP-76, IMP-77, IMP-78, IMP-79, IMP-80, IMP-81, IMP-82, IMP-83, IMP-84, IMP-85, IMP-86, IMP-87, IMP-88, IMP-89, IMP-90, IMP-91, IMP-92, IMP-93, IMP-94, IMP-95, IMP-96, IMP-97, IMP-98, IMP-99, IMP-100) and developing targeted primer sets for each family, allowing accurate detection of genetically diverse variants while maintaining a systematic and manageable assay structure
Solution Approach 2:
The patent creates a universal detection system that can identify multiple IMP families simultaneously using a single real-time PCR assay platform. The method employs multiple primer sets that can detect a broad range of IMP variants through a unified approach, making the system adaptable to different IMP families while maintaining operational simplicity
2Adaptability or versatility
If multiple primer sets are used to detect broad range of IMP variants, then the detection coverage is improved, but the assay complexity increases
Solution Approach 1:
The patent merges multiple detection capabilities into a single real-time PCR assay by combining multiple primer sets targeting different IMP families. This integration allows simultaneous detection of broad IMP variant ranges in one test, improving detection coverage while avoiding the need for separate assays for each IMP family
Solution Approach 2:
The assay design employs universal real-time PCR methodology that can accommodate multiple primer sets for different IMP families. This multi-functional approach enables a single platform to detect diverse IMP variants, expanding detection coverage without requiring multiple specialized systems
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables the accurate identification and classification of IMP carbapenemases, improving the detection of all IMP variants and enhancing antibiotic stewardship and infection control efforts by providing a more comprehensive understanding of gram-negative infections.
Implementation Method 1
utilize multiple primer sets to identify all IMP variants via real-time PCR assays
Implementation Method 2
The sets of PCR primers can also be coupled with a fluorescent dye to detect a broad range of the IMP variants
Data Source
AI summary
Systems and methods for detection and classification of IMP carbapenemases are described. In an aspect, sets of PCR primers associated with specific probes can be used to identify specific families of IMP β-lactamases and utilize multiple primer sets to identify all IMP variants via real-time PCR assays. The sets of PCR primers can also be coupled with a fluorescent dye to detect a broad range of the IMP variants.