PDMS Microwell Array for Cell Sorting via Needle Release
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current cell sorting technologies face challenges in efficiently isolating adherent cells without damaging them, particularly due to the need for expensive optical systems and the limitations of existing microcarrier materials like photoresist arrays which have autofluorescence issues and complex fabrication requirements.
Innovation Solution
A cell sorting apparatus using an elastomeric substrate with microwells and detachable rafts made from materials like polystyrene, where cells adhere and are released using a needle, eliminating the need for expensive optical systems and simplifying fabrication.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If photoresist arrays are used as microcarriers, then cell sorting can be performed on adherent cells, but autofluorescence interferes with detection and fabrication is complex
Solution Approach 1:
The patent uses inexpensive, disposable PDMS microwell arrays instead of expensive photoresist arrays. The PDMS substrates are readily available, low-cost, and can be discarded after use, eliminating the need for complex fabrication and cleaning procedures while avoiding autofluorescence issues
Solution Approach 2:
The patent changes the material parameter from photoresist to PDMS, which fundamentally alters the optical properties by eliminating autofluorescence and changes the surface properties to enable controlled cell adhesion and raft release
2Measurement precision
If laser-based release systems are used, then precise pallet release can be achieved, but the optical system is expensive and requires precise alignment and maintenance
Solution Approach 1:
The patent replaces the optical laser-based release system with a simple mechanical needle system. A thin needle physically pokes through the PDMS substrate to release individual rafts, eliminating the need for expensive lasers, beam splitters, mirrors, and complex alignment procedures while maintaining precise control over raft release
Solution Approach 2:
The PDMS substrate acts as a disposable mechanical component that can be easily pierced by a needle. After use, the entire substrate is discarded, eliminating the need for maintenance of complex optical systems
3Stability of the object's composition
If rigid substrates are used for cell carriers, then structural stability is maintained, but cell stress increases during release
Solution Approach 1:
The patent uses a flexible PDMS substrate instead of a rigid substrate. The elastomeric nature of PDMS allows the substrate to deform elastically when a needle pokes through it, reducing mechanical stress on the cells during the release process while maintaining structural integrity before release
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for efficient, cost-effective isolation of adherent cells with high viability and flexibility in cell sorting criteria, overcoming the limitations of existing methods by using a simple, inexpensive needle-based release mechanism and reducing cell stress.
Implementation Method 1
said carriers are configured to release from said substrate upon mechanical distortion of said substrate
Implementation Method 2
said carriers are coated with a biologically active molecule... selected from the group consisting of... a cell adhesion molecule
Data Source
Figure 1A~2D
Figure 3Ai~3E
Figure 4
AI summary
An apparatus for collecting or culturing cells or cell colonies includes: a common substrate formed from a flexible resilient polymeric material and having a plurality of wells formed therein; and a plurality of rigid cell carriers releasably connected to said common substrate, with said carriers arranged in the form of an array, and with each of the carriers resiliently received in one of the wells. A method of collecting or culturing cells or cell colonies with such an apparatus is carried out by depositing a liquid media carrying cells on the apparatus so that said cells settle on or adhere to said the carriers; and then (c) releasing at least one selected carrier having said cells thereon by gradual application of release energy to each carrier from the cavity in which it is received (e.g., by pushing with a probe).