Oligonucleotide Detection Kit for PDX Mouse Contamination

The patent applies preliminary action by establishing specific detection methods using real-time PCR and droplet digital PCR before cross-contamination significantly affects research results. The kit with specific primers and probes is prepared in advance to detect and quantify mouse stromal cell contamination, allowing researchers to take preventive measures before the contamination reaches levels that would compromise research accuracy.

The patent replaces mechanical or manual detection methods with molecular biological techniques. Instead of using traditional morphological or cultural methods to detect contamination, the invention employs PCR-based molecular detection systems that use specific primers and probes to identify and quantify mouse stromal cell DNA, providing a more sensitive and accurate substitution for conventional detection approaches.

The patent introduces specific primers and probes as intermediary elements that mediate between the complex requirement for high sensitivity/specificity and the need for operational simplicity. These molecular intermediaries selectively bind to mouse stromal cell-specific sequences, enabling the detection system to achieve high precision while maintaining relative operational simplicity through standardized PCR protocols.

The patent applies parameter changes by optimizing PCR reaction conditions, primer concentrations, and probe designs to achieve maximum sensitivity and specificity. By carefully adjusting these parameters, the detection system can distinguish even low levels of mouse stromal cell contamination from human cancer cells, transforming the detection capability from insufficient to highly accurate while maintaining procedural simplicity.