Albumin-Enriched Pea Protein Isolate via SHMP Extraction
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing methods for producing plant protein isolates are inefficient and do not maintain the native structure of proteins, resulting in suboptimal functionality and yield.
Innovation Solution
A process using sodium hexametaphosphate (SHMP) in a basic aqueous solution for extracting proteins from plant materials, followed by isoelectric precipitation, which includes steps of protein extraction, separation, and pH adjustment to produce a plant protein isolate.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Stability of the object's composition
If conventional methods are used to produce plant protein isolates, then the production process is simple, but the protein structure is damaged and functionality is reduced
Solution Approach 1:
The patent applies parameter changes by utilizing pH adjustment (isoelectric precipitation) and temperature control to extract proteins while maintaining their native structure. By changing the pH to the isoelectric point and controlling extraction temperature, the process achieves protein isolation without denaturation, resolving the contradiction between structural stability and process simplicity.
Solution Approach 2:
The patent uses an intermediary substance (salt or surfactant) as a mediator to facilitate protein extraction while preserving structure. The intermediary agent enables selective protein solubilization at neutral pH without direct harsh treatment of the protein, thus maintaining native conformation while achieving effective extraction.
2Productivity
If conventional extraction methods are used, then the process is economical, but the protein yield and functionality are suboptimal
Solution Approach 1:
The patent optimizes extraction parameters including pH (neutral to basic conditions), temperature (15-80°C), and time (5-60 minutes) to maximize protein yield while maintaining functionality. By systematically adjusting these parameters, the process achieves higher productivity without proportionally increasing manufacturing complexity.
Solution Approach 2:
The patent implements continuous protein extraction through prolonged exposure to optimal conditions (30-60 minutes), ensuring complete protein solubilization and maximizing yield. This continuous action allows efficient extraction without requiring multiple sequential steps, thereby maintaining ease of manufacture while improving productivity.
3Productivity
If proteins are extracted using harsh methods, then the extraction efficiency is high, but the protein functionality is compromised
Solution Approach 1:
The patent employs salt or surfactant as intermediary agents that facilitate efficient protein extraction through ionic interactions and micelle formation, respectively. These intermediaries enable high extraction efficiency without direct mechanical or thermal harshness, thus preserving protein functionality and reliability.
Solution Approach 2:
The patent utilizes controlled pH changes (to isoelectric point for precipitation) and temperature modulation to achieve efficient extraction while maintaining protein integrity. By changing parameters gradually and controllably rather than applying harsh conditions, the process achieves both high efficiency and functional reliability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The process maintains the native structure of proteins, improving functionalities such as solubility, foaming, and emulsifying capabilities, with a higher protein yield compared to conventional methods.
Implementation Method 1
a salt extraction step using sodium hexametaphosphate (SHMP) in a basic aqueous solution of plant product
Implementation Method 2
separating the protein solution from the unsolublized plant solids
Implementation Method 3
the separating step is by decantation
Implementation Method 4
precipitating protein from the protein solution by lowering pH to produce a plant protein isolate
Data Source
AI summary
The present disclosure provides an albumin enriched PPI extraction process and albumin enriched PPI produced by the process and use of albumin enriched PPI. The albumin enriched PPI extraction process comprises steps of a) protein extraction from aqueous solution comprising pea flour, b) separation of solids from the aqueous solution, producing a protein-rich liquid fraction, and c) precipitation of protein by use of Na, Ca, or Mg salt or combination thereof, thereby producing an albumin enriched PPI.