Peelable Layer Biomolecule Recovery Device
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Solution Overview
Problem
Current technologies fail to effectively combine multiplexed affinity-based screening and sample recovery of biomolecules, often resulting in contamination, loss, and destruction of samples, particularly in identifying and isolating biomolecules like aptamers with high binding affinity to proteins.
Innovation Solution
A device and method utilizing a peelable layer with a substrate immobilized on a support, where the peelable layer includes a target component, allowing for the isolation and recovery of biomolecules with affinity to the target component through a process involving binding, separation, and elution, enabling multiplexed screening and sample recovery without specialized equipment.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Loss of substance
If traditional scraping methods using tungsten needles are used to recover bound biomolecules from microarrays, then sample recovery is achieved, but sample contamination, loss and destruction occur
Solution Approach 1:
The device divides the microarray surface into multiple peelable layers that can be individually removed. Each layer contains specific bound biomolecules and can be separated without affecting other layers, enabling selective recovery of target samples without contamination or destruction.
Solution Approach 2:
The patent introduces a release coating layer between the substrate and the biomolecule-containing layer. This intermediary layer facilitates gentle separation of bound biomolecules from the microarray surface through peeling actions, replacing harsh scraping methods and preventing sample damage.
2Adaptability or versatility
If HPLC systems with multiple columns are used for affinity-based screening and sample recovery, then both screening and recovery are achieved, but the system becomes prohibitively expensive
Solution Approach 1:
The device combines affinity-based screening and sample recovery functions into a single integrated microarray platform. Multiple target components are immobilized on the same substrate, allowing parallel screening of multiple biomolecules while enabling recovery from the same device without requiring separate HPLC columns.
Solution Approach 2:
The microarray device serves multiple functions: it acts as both the screening platform for detecting biomolecule interactions and the recovery platform for isolating bound samples. The same substrate and target components are used for both detection and recovery purposes, eliminating the need for separate specialized equipment.
3Productivity
If microfluidic systems with heating electrodes or micromagnetic beads are used for aptamer selection and recovery, then affinity-based screening and recovery are achieved, but the systems require specialized equipment and cannot perform multiplexed selection simultaneously
Solution Approach 1:
The device transitions from single-point or single-line recovery methods to a two-dimensional array format. Multiple target components are arranged spatially on the substrate, allowing simultaneous multiplexed selection of multiple aptamers in parallel while maintaining simple manual operation without specialized equipment.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution allows for the efficient isolation, identification, storage, and recovery of biomolecules, preserving sample integrity and enabling multiplexed affinity-based screening, with the ability to store captured biomolecules for later elution and recovery, suitable for various applications including SELEX and drug screening.
Implementation Method 1
a target component immobilized on the substrate. The target component is a target biomolecule and/or a target biomaterial... effective for isolating and recovering a biomolecule having affinity to the target component
Data Source
AI summary
The present invention relates to a device for isolating and recovering a biomolecule from a test sample. The device includes a support and at least one peelable layer deposited on at least a portion of the support. The peelable layer includes a substrate having a target component immobilized on the substrate. The device is effective for isolating and recovering a biomolecule having affinity to the target component. The present invention also relates to systems and methods of using the device. The present invention also relates to a biomolecule elution strip and related methods.