PEG-Modified Py-C12 Fixation Composition for Fluid Retention

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Solution Overview

Problem

Pyrrolidine derivatives like Py-C12 cause increased fluid leakage and skin barrier permeability, leading to dehydration and unsightly epidermal lesions in fixed biological materials, compromising aesthetic and preservation quality.

Innovation Solution

Combining Py-C12 with polyethylene glycol (PEG) reduces fluid loss and skin barrier permeability, maintaining anti-microbial efficacy while improving aesthetic and preservation outcomes.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If pyrrolidine derivatives (Py-C12) are used for preservation and fixation of biological materials, then anti-microbial efficacy and penetration performance are improved, but fluid leakage and skin barrier permeability increase

Engineering Contradiction:

Improveanti-microbial efficacyVSAvoidfluid leakage

Core Design Contradiction:

ReliabilityVSLoss of substance

Solution Approach 1:

The patent introduces polyethylene glycol (PEG) as an intermediary substance that mediates between the pyrrolidine derivative and the biological material barrier. PEG reduces the harmful permeabilizing effect of Py-C12 on skin barriers while allowing the preservative to maintain its anti-microbial efficacy. The PEG acts as a protective intermediary that modifies the interaction between the fixation compound and biological barriers.

Inventive Principle:

Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent changes the physical-chemical parameters of the preservation composition by adding PEG, which alters the permeability characteristics of the solution. This parameter change reduces fluid leakage and epidermal lesion formation while maintaining the essential preservation function of the pyrrolidine derivative.

Inventive Principle:

Principle #35Parameter changes

2Speed

If pyrrolidine derivatives (Py-C12) are used for preservation and fixation of biological materials, then penetration performance is improved, but epidermal lesions and aesthetic quality worsen

Engineering Contradiction:

Improvepenetration performanceVSAvoidepidermal lesions

Core Design Contradiction:

SpeedVSObject-affected harmful factors

Solution Approach 1:

PEG serves as a protective intermediary that reduces the direct harmful interaction between Py-C12 and epidermal tissues. While Py-C12 maintains its penetration capability for effective preservation, PEG mitigates the formation of fluid-filled epidermal lesions by modulating the compound's interaction with skin barriers.

Inventive Principle:

Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent converts the harmful high permeability effect of Py-C12 into a beneficial controlled permeability by adding PEG. The excessive penetration that causes epidermal lesions is transformed into an optimized penetration profile that maintains preservation efficacy while reducing tissue damage and improving aesthetic outcomes.

Inventive Principle:

Principle #22Blessing in disguise (Convert harm into benefit)

3Reliability

If pyrrolidine derivatives (Py-C12) are used for preservation and fixation of biological materials, then biocidal efficacy is improved, but tissue dehydration worsens

Engineering Contradiction:

Improvebiocidal efficacyVSAvoidfluid loss

Core Design Contradiction:

ReliabilityVSLoss of substance

Solution Approach 1:

PEG acts as a hydrating intermediary that counteracts the dehydrating effect of Py-C12. While the pyrrolidine derivative maintains its biocidal activity against microorganisms, PEG retains moisture in the tissues, preventing excessive fluid loss and tissue dehydration during the preservation process.

Inventive Principle:

Principle #24Intermediary (Mediator)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

PEG significantly reduces epidermal lesions and fluid loss in Py-C12-fixed biological materials, enhancing fixation quality and retention without affecting biocidal activity.

Implementation Method 1

the addition of PEG significantly reduced the increased fluid leakage caused by compounds used for preservation and/or fixation such as Py-C12

Methodology Applied

Scientific EffectPermeability reduction:

Implementation Method 2

Use of polyethylene glycol for the reduction of permeability of barriers in biological materials, for gases and/or liquids

Methodology Applied

Scientific EffectPermeability reduction:

Data Source

PatentEP4627921A1Polyethylene glycol as an additive in compositions for preservation and/or fixation containing the pyrrolidine derivative py-c12

Publication Date: 2025.10.08 LICIT SOLUTIONS GMBH

Figure 1~2

AI summary

The present invention pertains to a combination of a pyrrolidine derivative, such as Py-C12 and polyethylene glycol, compositions comprising the above combination as well as the use of this combination and a method using the combination for the preservation and/or fixation of biological material. Furthermore, this invention pertains to the use of polyethylene glycol for the reduction of permeability of barriers in biological material.