Pentanediamine Fermentation CO2 Stripping Yield
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for producing 1,5-pentanediamine through whole-cell catalysis face challenges such as low yield, impurities like lysine, surface defects in polyamide resin materials, and high energy consumption due to the use of organic solvents, which increase production costs and operational difficulties.
Innovation Solution
A biocatalytic process involving E. coli BL21 (DE3) cells overexpressing lysine decarboxylase with pyridoxal phosphate, where carbon dioxide is removed by decompression and heating to adjust pH, followed by distillation under reduced pressure, reducing the need for strong bases and minimizing salt residue, thereby enhancing yield and purity of 1,5-pentanediamine.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If organic solvent extraction method is used to separate pentanediamine from fermentation broth, then separation can be achieved, but the purity of pentanediamine products is reduced by residual organic solvents
Solution Approach 1:
The patent extracts and removes organic solvents from the fermentation broth through evaporation and distillation processes. The organic phase containing pentanediamine is separated from the aqueous phase, and the organic solvent is then evaporated and distilled off to obtain high-purity pentanediamine free from residual solvent contamination.
Solution Approach 2:
The patent discards the organic solvent after extraction by evaporating and distilling it off from the organic phase. This allows the organic solvent to be removed and potentially recovered for reuse, while the pentanediamine remains as a high-purity product without residual solvent.
2Manufacturing precision
If organic solvents are used for extraction, then separation of pentanediamine is achieved, but the organic solvents have high odor, high toxicity, inflammable and explosive, which increase the operation difficulty of practical application
Solution Approach 1:
The patent replaces the mechanical handling and storage of large volumes of flammable organic solvents with a controlled evaporation and distillation process. The organic solvent is removed in a controlled manner through heating and vaporization, eliminating the need for continued handling of hazardous materials and reducing operational risks.
3Productivity
If organic solvents are used for extraction, then pentanediamine can be obtained, but organic solvents need to be recycled, which increase the process flow and energy consumption
Solution Approach 1:
The patent recovers organic solvents through evaporation and distillation processes. The solvent is evaporated from the organic phase and then distilled to separate and recover the pure solvent, which can be reused in subsequent extractions. This recovery process reduces the need for continuous fresh solvent addition and minimizes waste disposal requirements.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method increases the yield and purity of 1,5-pentanediamine, reduces the amount of salt residue and strong base usage, lowers production costs, and captures carbon dioxide emissions, making the process more economically and environmentally beneficial.
Implementation Method 1
removing carbon dioxide from the liquid obtained in the step (2) by decompressing and/or heating
Implementation Method 2
removing carbon dioxide from the liquid obtained in the step (2) by decompressing and/or heating
Implementation Method 3
extracting a composition containing 1,5-pentanediamine from the treatment liquid obtained in the step (3) by distillation under reduced pressure
Implementation Method 4
extracting a composition containing 1,5-pentanediamine from the treatment liquid obtained in the step (3) by distillation under reduced pressure
Data Source
Figure 1~2
Figure 3~4
AI summary
A method for fermentation-production of a pentanediamine, comprising: culturing a cell expressing a lysine decarboxylase to obtain a whole cell fermentation broth comprising a pentanediamine; and extracting the pentanediamine from the whole cell fermentation broth, and striping the whole cell fermentation broth of carbon dioxide contained therein before adding a strong base. The method greatly increases a production volume of the pentanediamine.