Pentas Flower Star Pattern via DFR Transcript Reduction

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Solution Overview

Problem

Current plant breeding methods fail to produce Pentas flowers with a unique anthocyanin pigment star pattern, characterized by a lack of pigment in the petal center and presence in the margins, which is not found in other varieties, limiting the creation of new flower colors and patterns.

Innovation Solution

The development of Pentas plants with a decreased level of dihydroflavonol reductase (DFR) transcripts, achieved through crossing and backcrossing techniques, results in flowers exhibiting an anthocyanin pigment star pattern, allowing for the introduction of this trait into different genetic backgrounds and the production of seeds that produce such plants.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If conventional plant breeding methods are used, then existing flower colors and patterns are maintained, but new unique flower patterns like the anthocyanin pigment star pattern cannot be produced

Engineering Contradiction:
Improveflower color and pattern diversityVSAvoidbreeding method complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent applies local quality by creating spatial variation in anthocyanin pigment deposition within the flower petals. Specifically, the petal center region exhibits reduced anthocyanin pigment deposition while the petal margins maintain normal pigment levels, resulting in the distinctive star pattern. This is achieved through localized downregulation of DFR gene expression in the petal center, allowing different regions of the same petal to have different pigment concentrations and thus different visual qualities.

Inventive Principle:
Principle #3Local quality

2Adaptability or versatility

If DFR transcript levels are decreased to create the star pattern, then unique flower patterns are achieved, but pigment deposition uniformity is reduced

Engineering Contradiction:
Improveflower pattern noveltyVSAvoidpigment deposition uniformity
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

The patent creates intentional local quality differences in pigment deposition by downregulating DFR expression specifically in the petal center while maintaining normal expression in the margins. This spatially differentiated gene expression pattern results in the desired star pattern with a lighter center and darker margins, accepting non-uniform pigment deposition as the means to achieve the novel aesthetic quality.

Inventive Principle:
Principle #3Local quality

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This approach enables the creation of novel Pentas varieties with unique flower patterns, enhancing ornamental qualities and attracting pollinators, while maintaining the plants' adaptability and desirable traits like disease resistance and environmental tolerance.

Implementation Method 1

The substantial lack of anthocyanin pigment is attributable to a decreased level of dihydroflavonol reductase (DFR) transcripts

Methodology Applied
Scientific EffectEnzyme catalysis: Enzyme

Data Source

PatentUS10631515B2Pentas flowers with a star pigment pattern
Publication Date: 2020.04.28 BALL HORTICULTURAL CO
  • US10631515B2 patent drawing

AI summary

The disclosure provides Pentas flowers comprising petals exhibiting an anthocyanin pigment star pattern, wherein said anthocyanin pigment star pattern is defined as comprising a substantial lack of anthocyanin pigment in the petal center and a presence of anthocyanin pigment in the petal margins. The disclosure also provides Pentas plants comprising a decreased level of dihydroflavonol reductase (DFR) transcripts resulting in an anthocyanin pigment star pattern and methods for producing a plant produced by crossing such plants with themselves or with another plant, such as a plant of another genotype. The disclosure further relates to seeds, plant parts, and plants produced by crossing Pentas plants of the disclosure with themselves or plants of a different genotype.