Pepper Hybrid Breeding with Single-Locus Trait Conversion
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Solution Overview
Problem
Existing pepper breeding methods struggle to develop uniform varieties with desirable traits such as herbicide tolerance, insect resistance, and disease resistance, often resulting in unpredictable performance due to genetic non-uniformity and the need for multiple generations of selfing and selection.
Innovation Solution
The development of pepper hybrid DRPB3992 and line SBO-XZ20-0212, which incorporates a single locus conversion through genetic engineering or backcrossing, conferring traits like herbicide tolerance, insect resistance, and disease resistance, and can be propagated as a homogeneous population with high genetic stability.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If traditional breeding methods are used to develop uniform varieties with desirable traits, then resistance to insects or disease and tolerance to environmental stress can be achieved, but the process requires multiple generations of selfing and selection, resulting in unpredictable performance due to genetic non-uniformity
Solution Approach 1:
The patent applies parameter changes by transitioning from traditional multi-generation breeding to a single-generation hybrid approach. By changing the breeding parameter from sequential selfing and selection cycles to a single cross-pollination event between homozygous inbred lines, the invention achieves uniform F1 hybrid progeny with desirable traits without requiring multiple generations, thereby resolving the contradiction between performance predictability and breeding time.
Solution Approach 2:
The patent employs preliminary action by first developing homozygous inbred lines through multiple generations of selfing and selection before creating the hybrid. This preliminary inbreeding process ensures that the parent lines are genetically uniform and stable, which then enables the production of uniform F1 hybrids in a single cross. The preliminary action of creating inbred parents resolves the contradiction by preparing the genetic foundation needed for predictable hybrid performance without requiring multiple hybrid generation cycles.
2Stability of the object's composition
If homozygous inbred plants are developed through self-pollination and selection, then uniform varieties can be produced, but the process becomes complex and time-consuming requiring multiple generations
Solution Approach 1:
The patent applies segmentation by dividing the breeding process into two distinct phases: (1) development of homozygous inbred parent lines through self-pollination and selection, and (2) single-generation cross-pollination to produce uniform F1 hybrids. This segmentation allows the complex inbreeding process to be confined to parent line development only, while the hybrid production itself is simplified to a single cross event, thereby reducing overall breeding complexity while maintaining genetic uniformity.
Solution Approach 2:
The patent uses preliminary action by completing the complex homozygous inbreeding process in advance to create stable parent lines before hybrid production. By performing the multi-generation selfing and selection as a preliminary step to establish inbred parents, the invention simplifies the subsequent hybrid breeding process to a single cross-pollination event, thereby reducing the complexity of the overall breeding program while achieving genetic uniformity in the F1 generation.
3Stability of the object's composition
If cross-pollination between homozygous plants is performed, then uniform hybrid population is produced, but the hybrid plants become heterozygous at many loci reducing stability
Solution Approach 1:
The patent applies local quality by creating homozygous inbred parent lines that are uniform at all loci, then crossing them to produce F1 hybrids that are heterozygous only at the specific loci where the parents differed. This localized heterozygosity at meaningful trait loci while maintaining homozygosity at all other loci through the inbred parent lines ensures both population uniformity and trait consistency in the hybrid generation, resolving the contradiction between uniformity and stability.
Data Source
AI summary
The invention provides seeds and plants of pepper hybrid DRPB3992 and pepper line SBO-XZ20-0212. The invention thus relates to the plants, seeds, plant parts, and tissue cultures of pepper hybrid DRPB3992 and pepper line SBO-XZ20-0212 and to methods for producing a pepper plant produced by crossing such plants with themselves or with another plant, such as a pepper plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to plants, seeds, plant parts, and tissue cultures of pepper hybrid DRPB3992 and pepper line SBO-XZ20-0212 comprising introduced beneficial or desirable traits.