Pepper Line SBY-XZ18-0028 Single-Locus Conversion for Population Uniformity
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Solution Overview
Problem
Existing pepper breeding methods struggle to develop uniform varieties with desirable traits such as herbicide tolerance, insect resistance, and disease resistance, as they often result in unpredictable genetic diversity and non-uniform populations.
Innovation Solution
The development of pepper line SBY-XZ18-0028, which includes a single locus conversion through genetic engineering techniques like CRISPR/Cas systems, allows for the introduction of specific traits like herbicide tolerance, insect resistance, and disease resistance, while maintaining genetic uniformity and stability.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If traditional breeding methods (pedigree breeding and recurrent selection) are used to develop inbred plants, then genetic diversity is increased through combining backgrounds from multiple plants, but the resulting populations become non-uniform with unpredictable performance
Solution Approach 1:
The patent extracts only the specific desirable trait (e.g., herbicide tolerance, disease resistance) from a donor plant and introduces it into a uniform inbred line through genetic engineering. This allows obtaining the beneficial trait without introducing unwanted genetic variation, thus maintaining population uniformity while achieving genetic improvement.
Solution Approach 2:
The invention applies local quality by making a targeted modification at a specific genetic locus rather than combining entire genetic backgrounds. The single locus conversion allows the plant to have uniform genetics throughout except for the specific improved trait, achieving both uniformity and targeted genetic enhancement.
2Adaptability or versatility
If multiple genetic loci are combined from different plants to achieve desirable traits, then trait diversity is improved, but manufacturing complexity and breeding time increase
Solution Approach 1:
The patent replaces the mechanical breeding process (crossing, selfing, and selecting over multiple generations) with genetic engineering techniques. Instead of physically crossing plants and waiting for trait segregation, the desired trait is directly introduced through transformation or genome editing, dramatically simplifying the process and reducing time.
Solution Approach 2:
The invention performs preliminary action by pre-selecting and introducing only the specific desirable trait(s) needed, rather than combining multiple genetic backgrounds and then selecting. The single locus conversion is done in advance through genetic engineering, eliminating the need for lengthy breeding programs to accumulate multiple traits.
3Reliability
If conventional breeding methods are used to introduce desirable traits, then trait improvement is achieved, but the breeding time and resource investment increase significantly
Solution Approach 1:
The patent replaces time-consuming mechanical breeding operations (multiple generations of crossing and selection) with direct genetic modification techniques. This substitution reduces the breeding cycle from several years to a single generation or less, while maintaining reliable trait improvement through precise genetic engineering.
Solution Approach 2:
The invention skips the lengthy intermediate breeding steps by directly introducing the desired trait through genetic engineering. Instead of going through multiple generations of crossing, selfing, and selection, the process rushes through to the final result by transforming the target plant with the specific gene or editing the specific locus directly.
Data Source
AI summary
The invention provides seeds and plants of pepper line SBY-XZ18-0028. The invention thus relates to the plants, seeds, plant parts, and tissue cultures of pepper line SBY-XZ18-0028 and to methods for producing a pepper plant produced by crossing such plants with themselves or with another plant, such as a pepper plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to plants, seeds, plant parts, and tissue cultures of pepper line SBY-XZ18-0028 comprising introduced beneficial or desirable traits.