Peptide Acetals for Protease Stability in Liquid Detergents
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Solution Overview
Problem
Existing enzyme stabilizers, particularly peptide aldehydes, are prone to inactivation due to chemical reactions, and there is a need for compounds that efficiently stabilize enzymes, especially proteases, while maintaining their inhibitory and stabilizing effects in liquid detergents.
Innovation Solution
The use of peptide acetals, which are in the acetal form of aldehydes, as enzyme inhibitors and stabilizers, particularly for proteases, that maintain their effectiveness during storage in liquid detergents and improve detergency.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If peptide aldehydes are used for enzyme stabilization, then enzyme stability is improved, but the stabilizers are prone to inactivation by chemical reactions
Solution Approach 1:
The patent uses peptide acetals as intermediary compounds that temporarily protect the aldehyde functionality. The acetal group acts as a mediator between the stabilizing aldehyde and the enzyme, providing stabilization while preventing direct chemical reactions that would inactivate the compound. This resolves the contradiction by introducing an intermediate protective structure.
Solution Approach 2:
The patent changes the chemical state of the aldehyde from its reactive free form to a protected acetal form. This parameter change in the molecular structure (from aldehyde to acetal) fundamentally alters the reactivity profile while maintaining the enzyme-stabilizing properties, thus resolving the contradiction between stability and chemical reactivity.
2Reliability
If peptide aldehydes are used to stabilize proteases, then protease activity is maintained, but the compounds may lose effectiveness during storage in liquid detergents
Solution Approach 1:
The patent applies preliminary protective action by converting the aldehyde to an acetal before storage. This preliminary modification prevents degradation during storage, ensuring the compound remains effective when needed. The acetal protection is applied in advance to prevent future inactivation.
Solution Approach 2:
The acetal group serves as a protective intermediary during storage, shielding the reactive aldehyde functionality from degradation. This intermediary structure maintains the compound's stability throughout storage while preserving its ability to stabilize proteases upon activation.
3Reliability
If existing stabilizers are used, then some enzyme protection is achieved, but there is insufficient efficiency in stabilizing enzymes, particularly proteases
Solution Approach 1:
The patent creates a composite molecular structure combining the peptide backbone with an acetal protecting group. This composite structure integrates the enzyme-binding properties of the peptide with the chemical stability of the acetal group, achieving superior stabilization efficiency compared to simple peptide aldehydes.
Solution Approach 2:
The patent optimizes the molecular parameters by modifying the aldehyde group to an acetal, which changes the chemical stability parameter while maintaining the biological activity parameters. This parameter optimization results in enhanced stabilization efficiency.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Peptide acetals effectively stabilize proteases and enhance the wash performance of detergents by maintaining their inhibitory and stabilizing effects, even in liquid formulations.
Implementation Method 1
The inventors have found that the acetal form of an aldehyde, particularly the acetal form of a peptide aldehyde, is itself effective as an enzyme inhibitor and stabilizer, particularly as a protease inhibitor and stabilizer
Data Source
AI summary
The present invention relates to a compound for stabilizing enzymes, the use of said compound for stabilizing an enzyme, a composition comprising said compound, a method of preparing the composition comprising said compound, a detergent composition comprising said compound and a method of preparing said compound.