Peptide Aldehyde Stabilizers for Liquid Detergent Enzymes

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Solution Overview

Problem

Existing liquid detergent compositions face challenges in stabilizing proteases and other enzymes, leading to instability and reduced effectiveness in aqueous environments.

Innovation Solution

Incorporating peptide aldehydes with a 'double N-capping group' as stabilizers, which enhance the stability of proteases and optional second enzymes in liquid detergent compositions, allowing them to maintain activity even when diluted with water.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Stability of the object's composition

If peptide aldehydes with double N-capping group are used as stabilizers, then enzyme stability is improved, but the complexity of the detergent composition increases

Engineering Contradiction:
Improveenzyme stabilityVSAvoidcomposition complexity
Core Design Contradiction:
Stability of the object's compositionVSDevice complexity

Solution Approach 1:

The invention changes the chemical structure parameters of the peptide aldehyde by introducing double N-capping groups (ureido, oxamide, thioureido, dithiooxamide, or thiooxamide groups) at specific positions. This structural modification transforms ordinary peptide aldehydes into effective enzyme stabilizers, resolving the contradiction by improving enzyme stability through targeted parameter changes rather than adding complex stabilizing systems

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The stabilizer combines multiple functional groups (peptide backbone, aldehyde group, and double N-capping group) into a single composite molecular structure. This composite approach allows the molecule to simultaneously bind to the enzyme and provide stabilization, achieving effective enzyme protection without requiring multiple separate additives that would increase formulation complexity

Inventive Principle:
Principle #40Composite materials

2Reliability

If enzymes are stabilized in concentrated form, then enzyme activity is maintained, but the detergent formulation becomes more viscous and harder to handle

Engineering Contradiction:
Improveenzyme activityVSAvoidhandling ease
Core Design Contradiction:
ReliabilityVSEase of operation

Solution Approach 1:

The peptide aldehyde with double N-capping group acts as an intermediary molecule that binds to the enzyme and prevents aggregation or degradation. This intermediary approach allows enzymes to remain stable in concentrated detergent formulations without requiring excessive water addition, thus maintaining both enzyme activity and manageable viscosity through the mediating stabilizer

Inventive Principle:
Principle #24Intermediary (Mediator)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The peptide aldehydes effectively stabilize proteases and other enzymes, maintaining their activity and functionality in liquid detergent formulations, particularly in aqueous solutions, thereby improving the stability and performance of the enzyme compositions.

Implementation Method 1

certain peptide aldehydes containing a 'double N-capping group' are effective as stabilizers for proteases in liquid enzyme formulations and in liquid detergent compositions

Methodology Applied
Scientific EffectEnzyme stabilization:

Data Source

PatentUS10577568B2Detergent composition
Publication Date: 2020.03.03 NOVOZYMES AS

AI summary

Peptide aldehydes containing a ureido group are effective as stabilizers for protease in a detergent composition, and as stabilizers for a second enzyme in a liquid composition with a protease.