Peptide Aldehyde Liquid Composition for Enzyme Stability
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Solution Overview
Problem
There is a challenge in creating homogenous liquid products containing enzyme stabilizers with limited solubility in water and water-miscible organic solvents that maintain stability during storage at various temperatures, particularly in providing storage-stable hydrolase compositions with sustained enzyme activity.
Innovation Solution
A solvent system comprising a peptide aldehyde, preferably a tripeptide aldehyde, combined with a mixture of organic solvents including 1,2-propane diol, which forms a homogenous liquid composition that retains stability and enzymatic activity over 50 days at different temperatures.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If enzyme stabilizers with limited solubility are used in water-containing hydrolase products, then enzyme stability during storage is improved, but the liquid composition becomes heterogeneous with visible particles and turbidity
Solution Approach 1:
The patent employs water-miscible organic solvents (1,2-propanediol, 1,3-propanediol, or glycerol) as intermediary substances to dissolve enzyme stabilizers that have limited solubility in water. These intermediaries bridge the solubility gap, allowing the stabilizers to remain in solution without causing heterogeneity, thus maintaining both enzyme stability and composition homogeneity simultaneously.
Solution Approach 2:
The patent changes the physical-chemical parameters of the solvent system by combining water with specific water-miscible organic solvents in optimized ratios. This parameter change modifies the solvent properties to enhance the dissolution capacity for enzyme stabilizers, enabling them to remain soluble at storage temperatures without causing precipitation or turbidity, thereby resolving the contradiction between stability and homogeneity.
2Reliability
If enzyme stabilizers are added to hydrolase products, then storage stability at elevated temperatures is improved, but solubility issues arise in water and water-miscible organic solvents
Solution Approach 1:
The patent creates a composite solvent system combining water with water-miscible organic solvents (1,2-propanediol, 1,3-propanediol, or glycerol). This composite material exhibits enhanced solubility properties compared to pure water, allowing enzyme stabilizers to dissolve effectively while maintaining the aqueous nature of the hydrolase product, thus resolving the solubility issue without compromising storage stability.
Solution Approach 2:
Water-miscible organic solvents serve as intermediary substances that facilitate the dissolution of enzyme stabilizers. These intermediaries enable the stabilizers to achieve adequate solubility in the aqueous environment, making the formulation process easier while preserving the storage stability benefits provided by the enzyme stabilizers.
3Ease of manufacture
If a single organic solvent is used to dissolve enzyme stabilizers, then solubility is improved, but storage homogeneity and stability are compromised
Solution Approach 1:
The patent merges multiple water-miscible organic solvents (1,2-propanediol, 1,3-propanediol, and/or glycerol) in specific combinations and ratios. This merging of solvents creates a synergistic effect where the composite solvent system provides both the solubility needed to dissolve enzyme stabilizers effectively and the storage stability required to maintain homogeneity at elevated temperatures, resolving the contradiction between ease of manufacture and reliability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution ensures the stability and homogeneity of the liquid composition, maintaining sufficient residual enzyme activity after storage at elevated temperatures for an extended period, addressing the solubility and storage stability issues of enzyme stabilizers.
Implementation Method 1
reversible enzyme inhibitors may be used, which are released in final application of an enzyme but are kept bound to the enzyme under storage conditions
Implementation Method 2
A solvent system comprising a peptide aldehyde, preferably a tripeptide aldehyde, combined with a mixture of organic solvents including 1,2-propane diol, which forms a homogenous liquid composition
Data Source
AI summary
A liquid composition, includingcomponent (a): at least one peptide aldehyde, andcomponent (b): at least two organic solvents, where one organic solvent is 1,2-propane diol and at least one further organic solvent is selected from the group consisting of diols other than 1,2-propane diol in a weight ratio of 1,2-propane diol: at least one diol other than 1,2-propane diol of 25:1 to 1:4.