Peptide Identification via Reflection Interference Spectroscopy
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Solution Overview
Problem
Current methods for determining the type of target peptides, such as those using mass spectrometry, are not efficient in identifying peptides due to complexity and require more straightforward and accurate approaches.
Innovation Solution
A device and method that involves obtaining and comparing dissociation rate constants, maximum layer thickness change amounts, and dissociation modes of target peptides with known peptides by immobilizing proteins on a solid phase carrier and using reflection interference spectroscopy to determine if the target peptide matches a known peptide.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If mass spectrometry is used to identify peptides, then peptide identification can be achieved, but the process becomes complex and less efficient
Solution Approach 1:
The patent extracts and measures only the essential binding characteristics (dissociation rate constant, layer thickness change, dissociation mode) of peptide-protein interactions, eliminating the need for complex mass spectrometry analysis while retaining sufficient information for accurate peptide identification and classification
Solution Approach 2:
The patent replaces the mechanical/mass spectrometry-based identification system with an optical measurement system (reflection interference spectroscopy) that measures physical changes in layer thickness and binding kinetics, simplifying the overall identification process while maintaining accuracy
2Reliability
If traditional peptide identification methods are used, then peptide type can be determined, but the method lacks straightforwardness and requires complex procedures
Solution Approach 1:
The patent performs preliminary classification of peptides into three types based on their dissociation modes (single dissociation, multiple dissociation, and stable binding modes) before detailed identification, providing a straightforward initial assessment that guides subsequent analysis and simplifies the overall determination process
Solution Approach 2:
The patent measures and utilizes only the critical partial information needed for peptide identification (three key binding characteristics) rather than attempting to analyze all possible peptide properties, making the procedure simpler and more straightforward while maintaining reliable identification
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for efficient and accurate identification of target peptides by simplifying the process and utilizing measurable physical changes to determine peptide type, enhancing the reliability of peptide identification.
Implementation Method 1
a solid phase carrier in which a target peptide is immobilized on a support through protein binding to a peptide
Implementation Method 2
measuring (i) changes in the layer thickness on the support of the protein immobilized carrier when the target peptide binds to the protein
Data Source
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AI summary
Disclosed is a device for determining a type of target peptide includes: an acquisition means that obtains first to third information on a target peptide contained in a test sample; a storage means that stores first to third information on a known peptide obtained using a standard sample containing the known peptide; and a control means that performs operations including: comparing the first to third information on the target peptide obtained from the test sample with the first to third information on the known peptide stored in the storage means; and determining whether or not the target peptide is the known peptide, wherein the first information is a dissociation rate constant when the target peptide dissociates from a protein, in a solid phase carrier in which the target peptide is immobilized on a support through the protein binding to a peptide, the second information is a maximum value of a layer thickness change amount on the support, and the third information is information indicating that a dissociation mode of a peptide and the protein is either a single dissociation mode or a multiple dissociation mode.