Peptide Nucleic Acid Probe Multiplex Detection

Resolve Bottlenecks,
Find Innovative SolutionsGenerate Solutions

Solution Overview

Problem

There is a lack of commercialized in vitro diagnostic tests for multiplex detection of BCR/ABL negative myeloproliferative neoplasm-associated gene mutations, and existing clinical trial data are insufficient, necessitating the development of a screening test that incorporates multiplex detection molecular genetic technology for clinical practice.

Innovation Solution

A peptide nucleic acid probe designed to complementarily bind to BCR/ABL negative myeloproliferative neoplasm-associated genes, including JAK2, MPL, and CALR, to enable multiplex detection of specific gene mutations such as JAK2 V617F, MPL W515L/K, and CALR L367fs*46/K385fs*47, using a composition and kit that minimizes interference between probes for precise detection.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If multiple probes are used for multiplex detection of gene mutations, then detection capability is improved, but interference between probes increases and detection precision deteriorates

Engineering Contradiction:

Improvemultiplex detection capabilityVSAvoiddetection precision

Core Design Contradiction:

Adaptability or versatilityVSMeasurement precision

Solution Approach 1:

The patent applies local quality by designing each PNA probe with specific structural characteristics - using peptide backbone instead of sugar-phosphate, and optimizing the probe length (8-20 nucleotides) and composition to minimize interference while maintaining high binding specificity to target mutation sites in JAK2, MPL, and CALR genes

Inventive Principle:

Principle #3Local quality

Solution Approach 2:

The patent changes key parameters of the probe system by using peptide nucleic acid (PNA) chemistry with neutral charge, adjusting probe length to 8-20 nucleotides, and optimizing hybridization conditions to achieve multiplex detection without probe interference, thereby improving both versatility and precision

Inventive Principle:

Principle #35Parameter changes

2Productivity

If conventional detection methods are used, then simplicity is maintained, but detection sensitivity and productivity are insufficient

Engineering Contradiction:

Improvedetection efficiencyVSAvoiddetection sensitivity

Core Design Contradiction:

ProductivityVSMeasurement precision

Solution Approach 1:

The patent replaces conventional mechanical/chemical detection systems with PNA-based molecular recognition system that utilizes complementary base pairing and melting temperature analysis, enabling rapid multiplex detection of multiple gene mutations simultaneously with high sensitivity and specificity

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent creates a universal PNA probe system that can detect multiple different gene mutations (JAK2 V617F, MPL W515L/K, CALR L367fs*46/K385fs*47) using the same basic platform and methodology, thereby improving productivity through multiplexing while maintaining high detection sensitivity

Inventive Principle:

Principle #6Universality (Multi-functionality)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The peptide nucleic acid probe enables rapid and accurate multiplex detection of BCR/ABL negative myeloproliferative neoplasm-associated gene mutations, facilitating disease diagnosis, follow-up, prognosis estimation, and treatment by minimizing interference and enhancing detection sensitivity and specificity.

Implementation Method 1

A peptide nucleic acid probe designed to complementarily bind to BCR/ABL negative myeloproliferative neoplasm-associated genes

Methodology Applied

Scientific EffectHybridization:

Data Source

PatentUS11028428B2Peptide nucleic acid probe for multiplex detection of BCR/ABL negative myeloproliferative neoplasm-associated gene mutations

Publication Date: 2021.06.08 THE CATHOLIC UNIV OF KOREA IND ACADEMIC COOP FOUND

AI summary

The present invention provides a peptide nucleic acid probe including any one or more selected from the group consisting of nucleic acid sequences represented by SEQ ID NOs: 7-10 and nucleic acid sequences complementary to the nucleic acid sequences, and manufactured so as to complementarily bind to a BCR/ABL negative myeloproliferative neoplasm-associated gene, thereby enabling multiplex detection of gene mutations; a composition for multiplex detection of gene mutations comprising the same; a kit; and a method for multiplex detection of BCR/ABL negative myeloproliferative neoplasm-associated gene mutations using the same. Therefore, since multiplex detection of BCR/ABL negative myeloproliferative neoplasm-associated gene mutations can be rapidly and accurately carried out by one detection process, BCR/ABL negative myeloproliferative neoplasm is effectively diagnosed, and thus the present invention can be useful for diagnosis, follow-up, prognosis estimation, and treatment of diseases.