Peptide Probe Biomolecule Separation and Detection
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Solution Overview
Problem
Current biological sensing technologies rely on polyclonal or monoclonal antibodies, which are costly and prone to misdiagnosis due to poor sensitivity, necessitating a rapid, accurate, and low-cost method for biomolecule separation.
Innovation Solution
A biological sensing device utilizing an amino acid sequence with high affinity to the UlaG protein, which is labeled on biomolecules, coupled with a signal-generating unit, allowing for the separation and detection of biomolecules through specific binding and signal generation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If polyclonal antibodies are used for detection, then the detection can be performed, but the cost is high and the sensitivity is poor leading to misdiagnosis
Solution Approach 1:
The patent uses a synthetic peptide probe instead of expensive monoclonal antibodies. The peptide probe is chemically synthesized, inexpensive to produce, and can be disposed of after use. This replaces the costly antibody-based detection system with a cost-effective alternative that maintains detection reliability.
Solution Approach 2:
The patent changes the detection parameter from antibody-antigen binding to peptide-protein binding. By designing a specific peptide sequence that binds to the UlaG protein with high affinity and specificity, the system achieves high detection accuracy without the cost and sensitivity issues of polyclonal antibodies.
2Reliability
If monoclonal antibodies are used for detection, then the detection can be performed, but the cost is high
Solution Approach 1:
The patent replaces expensive monoclonal antibodies with inexpensive synthetic peptide probes. The peptides are produced through chemical synthesis rather than complex biological processes, making them significantly cheaper while maintaining detection reliability through high-specificity binding to the target protein.
Solution Approach 2:
The patent creates a simplified copy of the antibody function using a peptide probe. Instead of using the complex antibody structure, a small peptide sequence is designed to replicate the specific binding capability, achieving the same detection function at much lower cost.
3Measurement precision
If polyclonal antibodies are used for detection, then the detection can be performed, but the sensitivity is poor leading to misdiagnosis
Solution Approach 1:
The patent changes the binding parameter from low-specificity polyclonal antibody binding to high-specificity peptide-protein binding. The peptide is designed to match a unique epitope on the UlaG protein, ensuring high detection sensitivity and eliminating misdiagnosis caused by cross-reactivity.
4Reliability
If conventional biological sensing technology is used, then detection can be performed, but it is not rapid enough
Solution Approach 1:
The patent replaces the complex mechanical and biological processes of antibody production and detection with a simpler chemical binding system. The peptide probe requires no complex preparation or biological systems, enabling rapid detection while maintaining reliability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables fast, accurate, and cost-effective detection of biomolecules, such as Streptococcus pneumoniae, with high sensitivity and selectivity, reducing the risk of misdiagnosis and unnecessary antibiotic administration.
Implementation Method 1
The amino acid sequence includes SEQ ID NO: 1 or SEQ ID NO: 2, and is for binding with UlaG protein labeled on a biomolecule
Data Source
AI summary
A biological sensing device and a method for separating a biomolecule are provided. The biological sensing device includes an amino acid sequence and a signal-generating unit. The amino acid sequence includes SEQ ID NO: 1 or SEQ ID NO: 2, and is for binding with UlaG protein labeled on a biomolecule. The signal-generating unit connects to the amino acid sequence.