Peptide Reagent for Optical Temperature Determination
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for determining the temperature of a sample during heating treatment and detecting target peptides in albumin-containing samples are inefficient, as they require direct contact with thermometers or non-contact methods, and struggle with accurately assessing temperature changes and peptide liberation.
Innovation Solution
A method involving mixing an albumin-containing sample with a peptide reagent comprising a peptide for temperature determination, which includes a first and second labeling substance, and detecting optical changes to determine if the temperature has reached a predetermined level, allowing for the liberation and detection of target peptides.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If direct contact temperature measurement methods are used, then temperature can be measured, but the measurement process becomes complex and requires additional equipment
Solution Approach 1:
The patent uses a peptide reagent as an intermediary substance that indirectly indicates temperature through optical changes. Instead of directly measuring temperature with thermometers, the peptide's binding state to albumin serves as a mediator that translates temperature information into detectable optical signals, simplifying the measurement system while maintaining accuracy
Solution Approach 2:
The patent replaces mechanical/physical temperature measurement systems (thermometers, sensors) with a chemical-biological system based on peptide-albumin binding equilibrium. The mechanical measurement apparatus is substituted by a chemical reaction system where the binding constant changes with temperature, converting a mechanical measurement problem into a chemical equilibrium problem that can be detected optically
2Productivity
If heating treatment is applied to liberate peptides from albumin, then target peptides can be detected, but temperature control becomes difficult without accurate measurement
Solution Approach 1:
The patent implements a feedback mechanism where the optical signal from the peptide reagent continuously monitors the temperature condition during heating. The binding state of the peptide reagent to albumin provides real-time feedback about whether the temperature has reached the required level for peptide liberation, allowing dynamic adjustment of heating to maintain optimal conditions
Solution Approach 2:
The peptide reagent serves a dual function: it both monitors temperature (through its temperature-dependent binding to albumin) and indicates when peptide liberation is complete. The system is self-regulating because the same molecular interaction that responds to temperature also provides the measurement signal, eliminating the need for separate measurement and control systems
3Temperature
If conventional temperature determination methods are used, then temperature can be assessed, but the process becomes time-consuming and complex
Solution Approach 1:
The patent utilizes optical changes (color/fluorescence changes) of the peptide reagent as a direct indicator of temperature. The peptide's binding state to albumin changes with temperature, resulting in detectable optical signal variations. This allows rapid visual or instrumental detection of temperature without time-consuming mechanical measurement procedures
Solution Approach 2:
The peptide reagent performs multiple functions simultaneously: it acts as a temperature sensor, a binding competitor for target peptides, and an optical signal generator. This multi-functionality consolidates what would otherwise require separate measurement and detection steps into a single integrated reagent system, dramatically reducing the time and complexity of temperature determination
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method enables simple and accurate determination of sample temperature and effective liberation of target peptides, avoiding direct contact with measurement tools and enhancing detection sensitivity through optical changes.
Implementation Method 1
a dissociation constant (Kd) of binding of the peptide for temperature determination to albumin is 500 μM or more
Implementation Method 2
detecting an optical change of the mixture; and determining whether the temperature of the mixture has reached the predetermined temperature based on the detection result of the optical change
Implementation Method 3
a sample containing a complex of a peptide and albumin is heated at a predetermined temperature to allow albumin to self-associate, thereby liberating the peptide from albumin
Implementation Method 4
the peptide for temperature determination comprises a first labeling substance and a second labeling substance
Data Source
AI summary
Disclosed is a method of determining whether a temperature of a sample has reached a predetermined temperature. The method comprises the steps of: mixing an albumin-containing sample with a peptide reagent comprising a peptide for temperature determination; heating the mixture; detecting an optical change of the mixture; and determining whether the temperature of the mixture has reached the predetermined temperature based on the detection result of the optical change. In the method, a dissociation constant (Kd) of binding of the peptide for temperature determination to albumin is 500 μM or more, and the peptide for temperature determination comprises a first labeling substance and a second labeling substance.