Peptide Synthesis Apparatus Using Co-localized tRNA and Assemblers
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Solution Overview
Problem
Current methods for peptide synthesis are limited in their ability to efficiently synthesize peptides with arbitrary amino acid sequences, particularly in a controlled and precise manner outside of cellular environments.
Innovation Solution
The method involves providing charged tRNA to specific locations and co-localizing them with biological assemblers, allowing for the sequential assembly of peptides with precise control over amino acid incorporation and temporal-spatial parameters, using fluid flow and monitoring systems to manage the process.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If traditional peptide synthesis methods are used, then peptide synthesis can be achieved, but the ability to synthesize peptides with arbitrary amino acid sequences in a controlled and precise manner is limited
Solution Approach 1:
The system divides the peptide synthesis process into discrete, controllable steps by providing charged tRNA to specific locations at specific time intervals. Each charged tRNA corresponds to a specific amino acid position in the target peptide sequence, allowing precise control over which amino acid is incorporated at each step. This segmentation enables arbitrary sequence synthesis while maintaining precision through temporal-spatial control.
Solution Approach 2:
The system performs preliminary actions by pre-positioning charged tRNA at specific locations before they are needed for peptide bond formation. The charged tRNA are provided to identifiable locations in advance, allowing the biological assemblers to have the correct amino acid ready for incorporation at the exact moment and position required, thus achieving both precision and versatility.
2Ease of operation
If cellular environments are used for peptide synthesis, then biological assembly can occur, but control over temporal-spatial parameters and amino acid incorporation is limited
Solution Approach 1:
The system extracts the essential peptide synthesis function from the complex cellular environment and creates a simplified, controlled in vitro system. By providing charged tRNA and biological assemblers in a controlled manner outside the cell, the system maintains the biological assembly capability while gaining precise control over temporal-spatial parameters and amino acid incorporation that is not available in natural cellular environments.
Solution Approach 2:
The system incorporates monitoring to track amino acid incorporation and peptide assembly progress. This feedback mechanism allows real-time observation and control of the synthesis process, enabling precise regulation of temporal-spatial parameters and ensuring reliable amino acid incorporation according to the target sequence.
3Productivity
If uncontrolled peptide synthesis is used, then peptide production can occur, but yield and specificity are reduced
Solution Approach 1:
The system merges the functions of amino acid delivery (charged tRNA) and peptide assembly (biological assemblers) into a coordinated, controlled process. By combining these elements and providing them to the same identifiable location at the same time, the system achieves both high yield and high specificity, as the merged system ensures that the correct amino acid is always available for incorporation into the correct position of the target peptide.
Data Source
AI summary
Methods, apparatus, systems, computer programs and computing devices related to biologically assembling and/or synthesizing peptides and/or proteins are disclosed.