pH-Buffering Reagent Layer for Durable Continuous Biosensing
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Solution Overview
Problem
Conventional biosensors struggle with continuous and direct monitoring of samples without continuous buffer solution feed, lacking pH buffering ability in their reagent layers.
Innovation Solution
A reagent layer containing a polymer with a proton accepting group in its repeating unit, combined with an oxidoreductase, provides pH buffering ability and maintains optimal reaction conditions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If a conventional biosensor without pH buffering ability in the reagent layer is used, then the device complexity is reduced, but the reliability deteriorates due to inability to maintain pH stability during continuous monitoring
Solution Approach 1:
The patent combines the pH buffering function with the reagent layer by incorporating a polymer containing proton-accepting groups (such as imidazole, pyridine, or carboxyl groups) directly into the reagent layer matrix. This merging of buffering capability into the existing reagent layer structure eliminates the need for separate buffering systems while maintaining pH stability during continuous analyte monitoring.
Solution Approach 2:
The reagent layer becomes self-sufficient by containing an embedded pH buffering system through the polymer with proton-accepting groups. This allows the reagent layer to autonomously maintain optimal pH conditions for enzyme activity without requiring external buffer solution feeds or additional pH control mechanisms, thereby improving reliability while avoiding increased device complexity.
2Reliability
If continuous buffer solution feed is implemented, then the pH buffering ability is improved, but the device complexity increases due to additional feed systems
Solution Approach 1:
The patent extracts the pH buffering function from the external buffer solution feed system and relocates it directly into the reagent layer through the incorporation of polymers with proton-accepting groups. This extraction eliminates the dependency on continuous buffer feeding while preserving the essential pH buffering ability, thereby reducing device complexity without sacrificing reliability.
Solution Approach 2:
The polymer containing proton-accepting groups acts as an intermediary buffering agent within the reagent layer. This intermediary substance provides pH buffering capacity locally at the enzyme active site without requiring external buffer solution feeds, thus maintaining pH buffering ability while avoiding the complexity of continuous feed systems.
3Duration of action of stationary object
If the reagent layer lacks pH buffering ability, then the ease of manufacture is improved, but the duration of action deteriorates due to enzyme deactivation
Solution Approach 1:
The patent merges the pH buffering function with the reagent layer fabrication process by incorporating polymers containing proton-accepting groups (such as polyvinylimidazole, poly-L-histidine, or carboxymethyl cellulose) directly into the reagent layer matrix during manufacturing. This integration extends sensor durability by preventing enzyme deactivation through pH stabilization while maintaining ease of manufacture through a unified fabrication approach.
Solution Approach 2:
The patent modifies the chemical composition parameters of the reagent layer by incorporating polymers with specific proton-accepting functional groups. This parameter change introduces pH buffering capability into the reagent layer, thereby extending the operational duration and durability of the biosensor without significantly complicating the manufacturing process, as these polymers can be integrated using standard reagent layer fabrication techniques.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The reagent layer maintains pH stability, preventing enzyme deactivation and enhancing sensor durability during continuous analyte monitoring.
Implementation Method 1
a polymer containing a proton accepting group in a repeating unit and having a pH buffering ability
Implementation Method 2
an oxidoreductase that oxidizes or dehydrogenates an analyte
Implementation Method 3
an oxidoreductase that oxidizes or dehydrogenates an analyte
Data Source
AI summary
In an embodiment of the present invention, there is provided a reagent layer comprising a polymer containing a proton accepting group in a repeating unit and having a pH buffering ability, and an oxidoreductase that oxidizes or dehydrogenates an analyte.