pH-Switchable Nucleic Acid Extraction Element for Rapid Purification
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Solution Overview
Problem
Current nucleic acid extraction methods, such as the magnetic bead method, are cumbersome, time-consuming, require low temperature storage, an external magnetic field, and generate waste, making them unsuitable for rapid and cost-effective nucleic acid detection.
Innovation Solution
A method and device for nucleic acid extraction using a nucleic acid extraction element made of polyacrylate, polyamide, polyester, or polyurethane that adsorbs nucleic acid in a pH 2-6 solution and releases it in a pH 8-12 solution, eliminating the need for low temperature, magnetic fields, and additional consumables, allowing extraction and purification in under 4 minutes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If the magnetic bead method is used for nucleic acid extraction, then the purity of extracted nucleic acid is high, but the operation time is long (at least 20 minutes) and the procedure is complicated
Solution Approach 1:
The patent changes the chemical parameters of the extraction system by using a solid-phase extraction element with specific pH characteristics. The extraction element adsorbs nucleic acid in acidic solution (pH 2-6) and releases it in basic solution (pH 8-12), eliminating the need for magnetic fields and complex multi-step procedures while maintaining high purity and reducing operation time to under 4 minutes
Solution Approach 2:
The patent extracts and removes the magnetic field requirement and complex reagent system from the extraction process. By using a solid-phase extraction element that can be simply rinsed with water, the method eliminates the need for magnetic beads, external magnetic fields, and multiple chemical reagents, thereby simplifying the procedure and reducing time
2Productivity
If the magnetic bead method is used for nucleic acid extraction, then the extraction efficiency is high, but additional consumables and equipment are required
Solution Approach 1:
The patent removes the requirement for magnetic beads, external magnetic fields, and complex reagent systems. The solid-phase extraction element can be directly rinsed with water to release nucleic acid, eliminating the need for magnetic separation equipment and multiple consumable reagents while maintaining high extraction efficiency
Solution Approach 2:
The extraction element is designed to be self-contained and can be directly rinsed with water to release the extracted nucleic acid. This self-service mechanism eliminates the need for external magnetic fields, complex washing buffers, and multiple step procedures, thereby reducing equipment and consumable requirements
3Reliability
If the magnetic bead method is used for nucleic acid extraction, then the purity is high, but low temperature storage is required
Solution Approach 1:
The patent changes the operational parameters by using a solid-phase extraction element that functions effectively at room temperature. The element adsorbs nucleic acid in acidic solution and releases it in basic solution without requiring low temperature storage or specialized equipment, thereby eliminating the temperature constraint while maintaining high purity
4Extent of automation
If the magnetic bead method is used for nucleic acid extraction, then the extraction can be automated, but the procedure is still complicated and time-consuming
Solution Approach 1:
The patent extracts and removes the complex multi-step procedure from the automation process. By using a solid-phase extraction element that can be simply rinsed with water, the method eliminates the need for multiple reagent additions, magnetic separations, and washing steps, thereby reducing extraction time to under 4 minutes while maintaining automation capability
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method achieves rapid nucleic acid purification comparable to existing methods, reducing operation time and waste while maintaining yield, and can be used for various applications including disease-related gene detection.
Implementation Method 1
a nucleic acid extraction element made of polyacrylate, polyamide, polyester, or polyurethane that adsorbs nucleic acid in a pH 2-6 solution
Implementation Method 2
releases it in a pH 8-12 solution
Data Source
AI summary
A nucleic acid extraction element is provided, which is made of one or more of polyacrylate, polyamide, polyester, polyurethane, and polylactic acid (PLA), where the extraction element adsorbs nucleic acid in a first solution with a pH value of 2 to 6, and releases the nucleic acid in a second solution with a pH value of 8 to 12. Therefore, the extraction element can be used for extraction and detection of the nucleic acid, greatly shorten the time for extraction, cleaning, and purification of the nucleic acid, simplify operating steps for extraction and purification of the nucleic acid, and reduce consumption of equipment for extraction and purification of the nucleic acid.