Maize Inbred PH1KN9 Marker-Assisted Trait Selection

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Solution Overview

Problem

Current maize breeding techniques face challenges in combining desirable traits such as disease resistance, drought tolerance, and uniformity, which are essential for efficient crop production and mechanical harvesting.

Innovation Solution

Development of a novel maize variety, PH1KN9, with specific breeding processes and genetic modifications to introduce desired traits through backcross conversion and transformation, enhancing resistance to various stresses and improving agronomic qualities.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional plant breeding methods are used to combine desirable traits, then the breeding process can be completed using conventional techniques, but the ability to effectively combine multiple desirable traits such as disease resistance, drought tolerance, and uniformity is limited

Engineering Contradiction:
Improveability to combine desirable traitsVSAvoideffectiveness of trait combination
Core Design Contradiction:
Adaptability or versatilityVSReliability

Solution Approach 1:

The patent employs molecular marker-assisted selection to change the parameter of trait selection from phenotypic observation to genotypic detection. By using DNA markers linked to desirable traits, breeders can reliably identify and combine multiple traits (disease resistance, drought tolerance, uniformity) in the PH1KN9 inbred line, overcoming the limitations of traditional visual selection methods.

Inventive Principle:
Principle #35Parameter changes

2Manufacturing precision

If conventional breeding techniques are used, then the breeding process is simpler, but the precision and uniformity of plant characteristics such as germination, stand establishment, growth rate, maturity, plant height and ear height are insufficient

Engineering Contradiction:
Improveuniformity of plant characteristicsVSAvoidcomplexity of breeding process
Core Design Contradiction:
Manufacturing precisionVSDevice complexity

Solution Approach 1:

The patent replaces mechanical/visual selection methods with molecular marker-assisted selection. Instead of manually evaluating plant characteristics like germination, stand establishment, and maturity, the invention uses DNA-based markers to precisely identify plants with desired genetic traits, achieving high uniformity without proportionally increasing process complexity.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent introduces molecular markers as intermediaries between the breeder and the target traits. These markers serve as proxies for desirable characteristics (disease resistance, drought tolerance, uniformity), allowing indirect but precise selection and combination of multiple traits in the PH1KN9 inbred line.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Productivity

If traditional breeding methods are employed, then the time to develop new varieties is shorter with simpler processes, but the yield and resistance to diseases, insects, heat and drought are not optimized

Engineering Contradiction:
Improveyield and resistanceVSAvoidtime to crop maturity
Core Design Contradiction:
ProductivityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by using molecular marker-assisted selection early in the breeding process to identify and select plants carrying desirable traits. This allows the PH1KN9 inbred line to accumulate multiple beneficial traits (disease resistance, drought tolerance, uniformity) more efficiently, reducing the overall time required to develop high-yielding, resilient varieties compared to traditional methods.

Inventive Principle:
Principle #10Preliminary action

Data Source

PatentUS9271461B1Maize inbred PH1KN9
Publication Date: 2016.03.01 PIONEER HI BREED INTERNATIONAL INC
  • US9271461B1 patent drawing

AI summary

A novel maize variety designated PH1KN9 and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing maize variety PH1KN9 with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH1KN9 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the variety PH1KN9 or a locus conversion of PH1KN9 with another maize variety.