PHA Extraction from Biomass Using Solvent Gelation Control

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Solution Overview

Problem

Current methods for extracting polyhydroxyalkanoates (PHAs) from biomass face challenges such as batch-to-batch variability, polymer degradation, and inefficient solvent recovery, particularly in industrial-scale mixed culture biomass processing, leading to inconsistent product quality and high production costs.

Innovation Solution

A method involving the use of PHA-poor solvents to extract PHAs from granulated biomass, maintaining specific temperature and shear stress conditions to prevent gelation, followed by controlled gelation and mechanical separation to recover high-quality PHA-rich solutions, optimizing extraction conditions for molecular weight and productivity.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Manufacturing precision

If conventional extraction methods are used to extract PHAs from biomass, then PHA recovery is achieved, but batch-to-batch variability leads to inconsistent product quality and high production costs

Engineering Contradiction:
Improveproduct quality consistencyVSAvoidproduction cost
Core Design Contradiction:
Manufacturing precisionVSProductivity

Solution Approach 1:

The patent applies parameter changes by systematically adjusting extraction temperature, solvent-to-biomass ratio, and extraction time to optimize PHA recovery. By controlling these parameters within specific ranges, the process achieves consistent product quality across different biomass batches while minimizing solvent consumption and production costs

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent implements feedback mechanisms through process monitoring and control systems that track extraction efficiency and product quality metrics. This allows real-time adjustments to extraction parameters, ensuring consistent PHA quality while optimizing resource utilization and reducing variability between batches

Inventive Principle:
Principle #23Feedback

2Productivity

If high temperature is used to extract PHA from biomass, then extraction efficiency is improved, but polymer degradation occurs

Engineering Contradiction:
Improveextraction efficiencyVSAvoidpolymer stability
Core Design Contradiction:
ProductivityVSReliability

Solution Approach 1:

The patent optimizes extraction temperature within a specific range (typically 60-80°C) to balance extraction efficiency with polymer stability. This controlled temperature parameter prevents thermal degradation of PHA while maintaining sufficient solubility for effective extraction, resolving the contradiction between productivity and reliability

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent uses specific solvents as intermediaries that enable PHA extraction at lower temperatures. These solvents facilitate mass transfer and PHA dissolution without requiring high thermal energy, thereby maintaining polymer integrity while achieving efficient extraction through chemical rather than purely thermal mechanisms

Inventive Principle:
Principle #24Intermediary (Mediator)

3Manufacturing precision

If solvent extraction is used to recover PHA from biomass, then PHA purification is achieved, but solvent consumption is high and recovery is inefficient

Engineering Contradiction:
ImprovePHA purificationVSAvoidsolvent consumption
Core Design Contradiction:
Manufacturing precisionVSLoss of substance

Solution Approach 1:

The patent implements solvent recovery and reuse mechanisms where extraction solvent is separated from the PHA product through filtration or centrifugation, then recovered and reused in subsequent extraction cycles. This significantly reduces solvent consumption while maintaining purification effectiveness, addressing the contradiction between manufacturing precision and substance loss

Inventive Principle:
Principle #34Discarding and recovering

Solution Approach 2:

The patent establishes continuous extraction and solvent recovery operations where spent solvent is immediately recovered and fed back into the extraction process. This continuous cycle minimizes solvent loss, maintains consistent purification quality, and improves overall process efficiency by eliminating idle time between extraction batches

Inventive Principle:
Principle #20Continuity of useful action

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This approach enables consistent product quality, reduced solvent consumption, and improved process economy by controlling molecular weight and minimizing polymer degradation, effectively addressing batch-to-batch variability and solvent recovery challenges.

Implementation Method 1

A method involving the use of PHA-poor solvents to extract PHAs from granulated biomass

Methodology Applied
Scientific EffectSolvent extraction: Liquid-Liquid Extraction

Implementation Method 2

maintaining specific temperature and shear stress conditions to prevent gelation, followed by controlled gelation and mechanical separation to recover high-quality PHA-rich solutions

Methodology Applied
Scientific EffectDissolution: Solvation

Implementation Method 3

followed by controlled gelation and mechanical separation to recover high-quality PHA-rich solutions

Methodology Applied
Scientific EffectGelation: Gel

Data Source

PatentEP2956493B1Process for the extraction of polyhydroxyalkanoates from biomass
Publication Date: 2023.01.04 PAQUES BIOMATERIALS HLDG BV
  • EP2956493B1 patent drawingFigure 1
  • EP2956493B1 patent drawingFigure 2
  • EP2956493B1 patent drawingFigure 3

AI summary

An industrial scale batch or semi-continuous batch PHA extraction process wherein the process operating conditions and control may be quantitatively tuned to batch-to-batch variations based on a practical bench scale assessment but more preferably by means of a calibrated chemometric assessment of the biomass quality. Tuning involves the selection of biomass loading conditions that are commensurate with process solvent and PHA type. Batch operating times and temperatures are matched to modelled and monitored extraction kinetics. PHA-rich solvent gelation is exploited by controlling PHA-rich solvent gel formation. In combination a process Is disclosed that permits for robust and consistent recovered product quality control while at the same time enabling to improve overall process economics.