Phospholipase Stabilizing Composition for Purification and Concentration

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Solution Overview

Problem

Existing methods fail to effectively prevent the inactivation of phospholipase during the purification and concentration steps in the enzyme production process, leading to loss of enzyme activity.

Innovation Solution

A composition comprising Tween 20 and/or sorbitol, or a combination of Tween 20 and glycerol, is added to a phospholipase solution to stabilize the enzyme, followed by concentration.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Manufacturing precision

If phospholipase undergoes purification and concentration steps, then product purity and concentration are improved, but enzyme activity is lost due to protein denaturation

Engineering Contradiction:

Improveproduct purityVSAvoidenzyme activity

Core Design Contradiction:

Manufacturing precisionVSReliability

Solution Approach 1:

The patent introduces a stabilization composition containing Tween 20 and sorbitol/glycerol as intermediary substances that mediate between the purification process and the phospholipase enzyme. These additives form a protective environment around the enzyme during concentration steps, preventing direct denaturation while allowing purification to proceed. This resolves the contradiction by enabling both high purity and maintained enzyme activity.

Inventive Principle:

Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent modifies the physical-chemical parameters of the purification environment by adding surfactants (Tween 20) and sugar alcohols (sorbitol/glycerol). These parameter changes create a stabilized microenvironment that prevents protein denaturation during concentration, allowing the system to achieve both high concentration and maintained catalytic activity simultaneously.

Inventive Principle:

Principle #35Parameter changes

2Productivity

If phospholipase undergoes concentration steps, then productivity is improved, but enzyme activity decreases due to inactivation

Engineering Contradiction:

Improveenzyme concentrationVSAvoidenzyme activity

Core Design Contradiction:

ProductivityVSReliability

Solution Approach 1:

The patent applies beforehand cushioning by pre-adding the stabilization composition (Tween 20 and sorbitol/glycerol) to the phospholipase solution before concentration steps. This creates a protective buffer that cushions the enzyme against denaturation stress during subsequent concentration operations, enabling high productivity while preserving enzyme activity through prior protective measures.

Inventive Principle:

Principle #11Beforehand cushioning (Prior cushioning)

Solution Approach 2:

The stabilization composition acts as an intermediary substance that facilitates the concentration process while protecting the enzyme. Tween 20 and sorbitol/glycerol create a protective matrix that allows water removal and enzyme concentration without direct exposure to denaturing conditions, resolving the contradiction between productivity gain and activity preservation.

Inventive Principle:

Principle #24Intermediary (Mediator)

3Reliability

If conventional additives are used to stabilize enzymes, then enzyme stability is improved, but the ability to prevent inactivation during purification and concentration remains insufficient

Engineering Contradiction:

Improveenzyme stabilityVSAvoidinactivation prevention capability

Core Design Contradiction:

ReliabilityVSManufacturing precision

Solution Approach 1:

The patent employs a composite stabilization system combining a surfactant (Tween 20) with sugar alcohols (sorbitol and/or glycerol). This composite approach creates synergistic effects where the surfactant provides surface protection while the sugar alcohols provide bulk stabilization, together offering superior protection during purification and concentration compared to single additives, thus resolving the insufficiency of conventional stabilization methods.

Inventive Principle:

Principle #40Composite materials

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The composition significantly increases the stability of phospholipase, enhancing product yield and economic feasibility by retaining enzyme activity during purification and concentration steps.

Implementation Method 1

enzymes have a protein structure, and thus can temporarily or permanently lose activity as the structure is sensitively altered by heat or pH

Methodology Applied

Scientific EffectProtein stabilization:

Implementation Method 2

Tween 20

Methodology Applied

Scientific EffectSurfactant action: Surfactant

Implementation Method 3

sorbitol, glycerol

Methodology Applied

Scientific EffectOsmotic stabilization: Osmosis

Data Source

PatentEP4711454A1Composition for stabilizing phospholipase and method for stabilizing phospholipase using same

Publication Date: 2026.03.18 CJ CHEILJEDANG CORP

AI summary

The present disclosure relates to a composition for stabilizing phospholipase and a method for stabilizing phospholipase.