Phospholipid Extraction from Undried Fish Roe

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Solution Overview

Problem

Current methods for obtaining omega-3 phospholipids from fish sources are inefficient, leading to low yields, high costs, and contamination issues due to heat-induced degradation and solvent concentration of pollutants, resulting in products with poor quality and safety concerns.

Innovation Solution

A method involving the use of undried fish roe or avian egg yolks contacted with a polar solvent, followed by membrane filtration to separate solvents and extract high omega-3 phospholipid compositions with reduced PCBs, PCDDs, PCDFs, and PAHs content, achieving a high DHA content and improved product stability.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If traditional drying and solvent evaporation methods are used to extract phospholipids, then lipid extraction efficiency is improved, but product quality deteriorates due to heat-induced oxidation and degradation

Engineering Contradiction:
Improvelipid extraction efficiencyVSAvoidproduct quality
Core Design Contradiction:
ProductivityVSManufacturing precision

Solution Approach 1:

The patent changes the physical state parameter of the starting material from dried to undried fish roe, eliminating the need for high-temperature drying. This parameter change prevents thermal degradation of omega-3 fatty acids while maintaining extraction efficiency through optimized solvent contact with fresh tissue.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent extracts phospholipids directly from undried fish roe using polar solvents before oxidative degradation can occur. By taking out the desired phospholipid components through selective solvent extraction rather than thermal processing, the method preserves product quality while achieving efficient separation.

Inventive Principle:
Principle #2Taking out (Extraction)

2Speed

If solvent evaporation by heat is used to concentrate lipid extracts, then extraction speed is improved, but harmful factors increase due to concentration of pollutants

Engineering Contradiction:
Improveextraction speedVSAvoidpollutant concentration
Core Design Contradiction:
SpeedVSObject-generated harmful factors

Solution Approach 1:

The patent uses selective polar solvent extraction to take out phospholipids from fish roe while leaving non-polar pollutants behind. This selective extraction mechanism separates desired components from contaminants without requiring high-temperature evaporation that would concentrate harmful substances.

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The patent exploits the different polarity characteristics of phospholipids versus pollutants to achieve local quality differentiation in the extraction process. Polar solvents selectively dissolve phospholipid components while non-polar contaminants remain in the solid matrix, enabling separation based on chemical property differences.

Inventive Principle:
Principle #3Local quality

3Ease of manufacture

If fish roe is dried before extraction to remove water, then extraction simplicity is improved, but phospholipid availability decreases due to binding with tissue components

Engineering Contradiction:
Improveextraction simplicityVSAvoidphospholipid availability
Core Design Contradiction:
Ease of manufactureVSQuantity of substance

Solution Approach 1:

Instead of drying fish roe before extraction as in conventional methods, the patent inverts the sequence by extracting phospholipids from undried roe. This inversion prevents phospholipid binding to tissue proteins that occurs during drying, maintaining higher availability and extractability of the target compounds.

Inventive Principle:
Principle #13The other way round (Inversion)

Solution Approach 2:

The patent performs extraction as a preliminary action before drying or freezing that would cause phospholipid binding. By conducting the extraction process on fresh, undried tissue, the method ensures maximum phospholipid availability before any structural changes occur in the tissue matrix.

Inventive Principle:
Principle #10Preliminary action

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The process yields a high-quality, stable phospholipid composition with enhanced DHA content, reduced pollutant levels, and improved safety and environmental profiles, suitable for dietary supplements and food products.

Implementation Method 1

contacting undried fish roe or avian egg yolks with a polar solvent, extracting a lipid fraction from the fish roe or the avian egg yolks

Methodology Applied
Scientific EffectLiquid-liquid extraction: Liquid-Liquid Extraction

Implementation Method 2

subjecting the solution to membrane filtration to provide a retentate

Methodology Applied
Scientific EffectMembrane filtration: Semipermeable Membrane

Implementation Method 3

removing water from the retentate to provide a solid phospholipid composition

Methodology Applied
Scientific EffectEvaporation: Evaporation

Data Source

PatentEP3271054B1Methods for obtaining phospholipids and compositions thereof
Publication Date: 2021.02.17 ARCTIC NUTRITION
  • EP3271054B1 patent drawingFigure 1
  • EP3271054B1 patent drawingFigure 2
  • EP3271054B1 patent drawingFigure 3

AI summary

The invention provides methods for obtaining lipid compositions comprising phospholipids having a high docosahexaenoic acid (DHA) content, which compositions are preferably extracted from natural sources such as fish roe. The lipid compositions are excellent sources of highly bioavailable DHA and they can be used in oral delivery vehicles, dietary supplements, functional foods, and the like.