Phosphor Nanoparticle Labeling Agent Spacer Design
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Conventional phosphor-integrated nanoparticle labeling agents face challenges in achieving sufficient signal intensity with low concentrations while minimizing non-specific adsorption, leading to increased noise in immunofluorescent staining.
Innovation Solution
A phosphor-integrated nanoparticle labeling agent is developed, comprising a probe biological substance linked to a first binding group via a polymer-derived spacer of specific length (30 Å to 1,000 Å) that binds to a biomolecule, and a phosphor-integrated nanoparticle with a second binding group capable of binding to the first binding group, optimizing signal intensity and reducing non-specific adsorption.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Illumination intensity
If the concentration of probe biological substance and phosphor-integrated nanoparticle is increased to improve signal intensity, then the fluorescence signal intensity is improved, but non-specific adsorption increases leading to increased noise
Solution Approach 1:
The patent optimizes the concentration parameters of both the probe biological substance and phosphor-integrated nanoparticle to achieve sufficient signal intensity while minimizing non-specific adsorption. By carefully controlling these concentration parameters, the invention resolves the contradiction between obtaining strong fluorescence signals and reducing background noise.
2Object-affected harmful factors
If the concentration of phosphor-integrated nanoparticle is decreased to reduce non-specific adsorption, then noise is reduced, but signal intensity becomes insufficient
Solution Approach 1:
The patent determines optimal concentration parameters for phosphor-integrated nanoparticles that balance signal intensity and noise reduction. Through parameter optimization, the invention achieves low noise levels while maintaining sufficient fluorescence signal strength for reliable detection.
3Object-affected harmful factors
If the length of the spacer is increased to reduce non-specific adsorption, then noise is reduced, but the binding efficiency may be affected
Solution Approach 1:
The patent optimizes the spacer length parameter to achieve the right balance between reducing non-specific adsorption and maintaining effective binding. By carefully selecting the spacer length, the invention minimizes noise while preserving the reliability of specific biomolecule binding.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution enables sufficient signal intensity at low phosphor-integrated nanoparticle concentrations and reduces noise in immunofluorescent staining, enhancing the detection sensitivity and quantitative accuracy of biomolecules.
Implementation Method 1
phosphor-integrated nanoparticle labeling agent
Data Source
AI summary
Provided are: a phosphor-integrated nanoparticle labeling agent which is capable of yielding a sufficient signal intensity even when its final concentration in an immunofluorescent staining reaction system is low (e.g., 0.02 nM) and enables to obtain an immunofluorescently stained image with reduced noise by inhibiting non-specific adsorption of a probe biological substance and a label to substances other than a detection subject; and an immunostaining method using the same. The phosphor-integrated nanoparticle labeling agent is a set which includes: a probe biological substance 3, which is linked to a first binding group substance A via a first hydrophilic polymer-derived spacer 1 having a length of 30 Å to 1,000 Å and specifically binds to a biomolecule 2; and a phosphor-integrated nanoparticle 5, which has a second binding group substance B capable of specifically binding to the first binding group substance A.