Photobioreactor System for Algae Culture Environmental Simulation

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Solution Overview

Problem

Conventional bioreactors fail to translate lab-grown algal strains' growth into outdoor productivity due to the difficulty in replicating natural conditions, leading to resource wastage in outdoor testing.

Innovation Solution

A photobioreactor system with advanced control modules for light, temperature, gas sparging, pH, and dilution, featuring a modular cap design, non-reflective surfaces, metal-free pathways, and electronic modularity to emulate outdoor pond environments, allowing for precise control of microorganism culture conditions.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional bioreactors are used to grow algal strains in the lab, then microorganism culture can be maintained, but the growth conditions cannot be translated to outdoor productivity due to inability to replicate natural conditions

Engineering Contradiction:
Improvegrowth consistencyVSAvoidenvironmental condition replication
Core Design Contradiction:
ReliabilityVSAdaptability or versatility

Solution Approach 1:

The system dynamically adjusts multiple environmental parameters (light intensity, temperature, pH, dissolved oxygen, liquid volume) to replicate natural outdoor pond conditions. This allows the bioreactor to maintain reliable growth while adapting to simulate varying natural environments, resolving the contradiction between growth consistency and environmental replication capability.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The bioreactor integrates multiple control functions (lighting control, temperature control, pH control, gas sparging, dilution control) into a single system that can replicate various natural conditions. This multi-functional design enables the system to maintain reliable growth while adapting to different environmental scenarios, addressing both reliability and adaptability requirements.

Inventive Principle:
Principle #6Universality (Multi-functionality)

2Measurement precision

If lab environments are used to control conditions and parameters, then scientifically significant data can be captured, but the conditions cannot reliably emulate natural conditions

Engineering Contradiction:
Improvedata accuracyVSAvoidnatural condition emulation
Core Design Contradiction:
Measurement precisionVSAdaptability or versatility

Solution Approach 1:

The system uses sensors to continuously monitor environmental parameters (light intensity, temperature, pH, dissolved oxygen, liquid volume) and feeds this information back to control modules that automatically adjust conditions. This feedback mechanism ensures both precise measurement capability for data accuracy and the ability to dynamically emulate natural condition variations.

Inventive Principle:
Principle #23Feedback

Solution Approach 2:

The system dynamically modifies environmental parameters based on target natural condition profiles, allowing it to maintain measurement precision while accurately emulating the variable conditions found in outdoor ponds, thus resolving the contradiction between data accuracy and natural condition replication.

Inventive Principle:
Principle #35Parameter changes

3Reliability

If outdoor testing is performed to verify algal strain productivity, then natural condition performance can be assessed, but resources are wasted due to lack of prior lab simulation capability

Engineering Contradiction:
Improveproductivity predictionVSAvoidresource wastage
Core Design Contradiction:
ReliabilityVSLoss of substance

Solution Approach 1:

The system performs preliminary testing in the lab by replicating natural outdoor conditions in the bioreactor, allowing assessment of algal strain productivity before outdoor deployment. This preliminary action predicts performance reliably and reduces resource wastage by identifying promising strains beforehand, eliminating the need for extensive trial-and-error outdoor testing.

Inventive Principle:
Principle #10Preliminary action

4Manufacturing precision

If a photobioreactor system with multiple control modules is implemented, then precise environmental control is achieved, but system complexity increases

Engineering Contradiction:
Improveenvironmental control precisionVSAvoidsystem structure
Core Design Contradiction:
Manufacturing precisionVSDevice complexity

Solution Approach 1:

The system combines multiple control functions (lighting, temperature, pH, gas sparging, dilution) and monitoring capabilities into an integrated photobioreactor platform. This merging approach achieves precise environmental control across multiple parameters while managing system complexity through unified design, resolving the contradiction between control precision and structural complexity.

Inventive Principle:
Principle #5Merging (Combining)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The system effectively simulates natural outdoor conditions, enabling accurate long-term maintenance of culture solutions and improving the growth of microorganisms by maintaining precise control over environmental parameters, thus reducing the need for outdoor testing.

Implementation Method 1

a lighting element produces stable light intensity range

Methodology Applied
Scientific EffectLight emission: Light Emitting Diode

Implementation Method 2

uses a magnetic stirrer

Methodology Applied
Scientific EffectMagnetic stirring: Electromagnetic Stirring

Implementation Method 3

a temperature control module, wherein the control is located at the bottom of the sample vessel

Methodology Applied
Scientific EffectThermoelectric cooling: Peltier Effect

Implementation Method 4

a gas sparging control module

Methodology Applied
Scientific EffectGas sparging: Sparging

Implementation Method 5

The tubing may include one or more peristaltic pumps

Methodology Applied
Scientific EffectPeristalsis: Peristalsis

Implementation Method 6

a weighing module, wherein the weighing module monitors volume

Methodology Applied
Scientific EffectStrain gauge measurement:

Data Source

PatentUS20240409872A1Photobioreactor methods and systems for accurate environmental maintenance of biological cultures
Publication Date: 2024.12.12 COLORADO SCHOOL OF MINES
  • US20240409872A1 patent drawing
  • US20240409872A1 patent drawing
  • US20240409872A1 patent drawing

AI summary

Methods and systems for controlling microorganism culture conditions are disclosed. The system may comprise a sample vessel and plumbing free metal parts, and use a modular cap design to adapt to a multitude of analytical probes, and possess a square profile with non-reflective surfaces. The system may also include stirrer; a lighting control module, a lighting element may produce stable light intensity range; a temperature control module that may be located at the bottom of the sample vessel; a pH control; a gas sparging control module; a dilution control module; a liquid storage system; an electronic modularity; a weighing module; and a scheduling system. In many embodiments of the system and method, the pH control may hold the pH within 0.1 of a target pH, the weighing module may monitor volume, and the scheduling system may allow the creation of user defined events and set points.