Photostable Detectable Compounds for Fluorescence Stability
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Solution Overview
Problem
Fluorescent dyes used in biological applications face limitations due to photobleaching and phototoxicity when exposed to illumination sources, affecting their performance in microscopy, cytometry, and DNA sequencing.
Innovation Solution
Development of photostable detectable compounds with specific fluorescent dye moieties and bioconjugate reactive groups, covalently linked to biomolecules, which maintain fluorescence intensity over time and reduce toxicity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Duration of action of moving object
If fluorescent dyes are used for extended illumination in biological applications, then imaging and detection capabilities are achieved, but photobleaching occurs reducing fluorescence intensity over time
Solution Approach 1:
The patent modifies the chemical structure of fluorescent dyes by introducing specific substituent groups (e.g., fluorinated alkyl groups, sulfonate groups) and adjusting molecular parameters to enhance photostability. These parameter changes in the dye structure directly address the photobleaching issue while maintaining fluorescence properties.
Solution Approach 2:
The invention creates composite fluorescent probe structures by combining fluorescent dye moieties with bioconjugate reactive groups and linker moieties. This composite approach integrates multiple functional components into a single molecule that simultaneously provides fluorescence, target binding, and enhanced stability against photobleaching.
2Duration of action of moving object
If fluorescent dyes are used for extended illumination, then imaging capability is maintained, but phototoxicity increases causing damage to biological samples
Solution Approach 1:
The patent optimizes the chemical parameters of fluorescent dyes by incorporating specific substituent groups that reduce reactive oxygen species generation and minimize phototoxic effects on biological samples, allowing longer imaging durations without sample damage.
Solution Approach 2:
The invention introduces intermediary protective groups and linker moieties that act as buffers between the fluorescent dye and the biological sample, reducing direct phototoxic interactions while maintaining the imaging function.
3Illumination intensity
If conventional fluorescent dyes are used, then initial fluorescence intensity is achieved, but signal stability deteriorates over time due to photobleaching
Solution Approach 1:
The patent systematically modifies molecular parameters including substituent types, linker lengths, and dye core structures to achieve optimal balance between high initial fluorescence intensity and long-term signal stability, preventing photobleaching-induced signal deterioration.
Solution Approach 2:
The invention applies local structural modifications at specific positions within the fluorescent molecule (e.g., adding stabilizing groups at particular locations on the dye core) to enhance overall photostability while preserving the fluorescence-generating regions of the molecule.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The compounds exhibit stable fluorescence over extended exposure to illumination, minimizing photobleaching and phototoxicity, thereby enhancing imaging and analysis in biological applications.
Implementation Method 1
R1 is a fluorescent dye moiety... detecting a light emission from the detectable moiety
Data Source
AI summary
Disclosed herein, inter alia, are detectable compounds including a triplet state quencher and the method of use thereof.