PHWVZ Doubled Haploid Maize Line for Transgene Introgression
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
The existing maize transformation technology using the Hi-II line is hindered by its hybrid nature, making it difficult to achieve a uniform, homozygous background for transgene development, and it lacks the quality genetics present in elite inbreds, requiring labor-intensive backcrossing to remove deleterious genomes.
Innovation Solution
Development of a novel double haploid maize line, PHWVZ, which is homozygous and has elite genotype characteristics, maintaining high transformability and culturability, allowing for efficient introgression of transgenes into an elite background through backcross conversion and transformation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If Hi-II maize line is used for transformation, then transformability and culturability are improved, but homozygosity and elite genotype quality deteriorate
Solution Approach 1:
The patent applies preliminary action by creating doubled haploid lines before transformation. The Hi-II line is crossed with elite inbred lines, and doubled haploid progeny are generated and screened for homozygosity and transformation capability. This preliminary creation of homozygous lines with elite genetics before the transformation process resolves the contradiction by ensuring both transformability and homozygosity are achieved in the same line.
2Ease of manufacture
If Hi-II maize line is used for transformation, then transformability is improved, but elite genotype quality deteriorates, requiring labor-intensive backcrossing
Solution Approach 1:
The patent performs preliminary action by pre-crossing Hi-II with elite inbred lines and selecting doubled haploid progeny that already possess elite genotype characteristics. This eliminates or significantly reduces the need for subsequent labor-intensive backcrossing to remove deleterious Hi-II genome, thereby saving time while maintaining transformability.
Solution Approach 2:
The patent extracts the desirable elite genotype characteristics from elite inbred lines and combines them with the transformability of Hi-II through controlled crossing and doubled haploid selection. This extraction and combination approach removes the need for extensive backcrossing to eliminate unwanted Hi-II traits.
3Ease of manufacture
If non-homozygous plants are used for transgene development, then transformability is maintained, but determination of transgene effects becomes difficult
Solution Approach 1:
The patent applies local quality by ensuring that the genetic background is uniformly homozygous while allowing the transgene to be the variable element. The doubled haploid lines provide a homogeneous genetic background, which enhances the precision of measuring transgene effects by eliminating genetic variability as a confounding factor.
Data Source
AI summary
A novel double haploid maize line designated PHWVZ and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing double haploid maize line PHWVZ with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PHWVZ through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the double haploid line PHWVZ or a trait conversion of PHWVZ with another maize line. Inbred maize lines derived from double haploid maize line PHWVZ, methods for producing other inbred maize lines derived from double haploid maize line PHWVZ and the inbred maize lines and their parts derived by the use of those method.