Phycocyanin Stabilization in Cosmetic Formulations

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Solution Overview

Problem

Phycocyanin is sensitive to temperature, pH changes, and oxidation, making it challenging to stabilize in cosmetic and dermatological formulations without affecting its biological properties, which are beneficial for skin aging and wound healing.

Innovation Solution

A composition comprising phycocyanin combined with resveratrol, ascorbyl methylsilanol pectinate, tocopherol or its acetate, capryloyl glycine, pH adjusters, and a citrate phosphate buffer in a topically acceptable medium, which maintains phycocyanin's stability and biological activity.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If phycocyanin is added to cosmetic formulations, then beneficial biological properties for skin aging and wound healing are provided, but stability against temperature, pH changes, and oxidation is compromised

Engineering Contradiction:
Improvebiological activityVSAvoidphycocyanin stability
Core Design Contradiction:
ReliabilityVSStability of the object's composition

Solution Approach 1:

The patent uses multiple stabilizing agents (polyphenols, vitamins, amino acids) as intermediaries to protect phycocyanin from environmental stressors. These mediators form protective complexes or create a favorable microenvironment that shields the phycocyanin-protoporphyrin complex from oxidation, temperature changes, and pH variations while allowing the biological activity to remain intact.

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The invention creates a composite formulation system where phycocyanin is combined with multiple stabilizing components (polyphenols like resveratrol and tannic acid, vitamins like ascorbic acid and tocopherol, and amino acids like capryloyl glycine). This composite approach provides synergistic protection against multiple degradation pathways simultaneously, maintaining both stability and biological functionality.

Inventive Principle:
Principle #40Composite materials

2Stability of the object's composition

If phycocyanin is stabilized using conventional methods, then stability is improved, but biological properties may be adversely affected

Engineering Contradiction:
Improvephycocyanin stabilityVSAvoidbiological activity
Core Design Contradiction:
Stability of the object's compositionVSReliability

Solution Approach 1:

The patent optimizes specific parameters including pH control (using citrate-phosphate buffer systems), temperature control during storage and application, and concentration ratios of stabilizing agents to phycocyanin. These parameter optimizations ensure stability is achieved through environmental control rather than chemical modification that could alter biological activity.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The stabilizing agents act as intermediaries that protect phycocyanin without forming irreversible complexes that would alter its structure. The polyphenols and antioxidants create a protective environment that allows phycocyanin to maintain its native conformation and biological functionality while being shielded from degradation.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Stability of the object's composition

If multiple stabilizing agents are added to protect phycocyanin, then stability against degradation is improved, but formulation complexity increases

Engineering Contradiction:
Improvephycocyanin stabilityVSAvoidformulation complexity
Core Design Contradiction:
Stability of the object's compositionVSDevice complexity

Solution Approach 1:

The selected stabilizing agents serve multiple functions simultaneously. For example, ascorbic acid acts as both an antioxidant protecting against oxidation and a pH buffer component. Tocopherol provides both antioxidant protection and membrane stabilization. This multi-functionality reduces the need for separate additive systems and simplifies the overall formulation approach.

Inventive Principle:
Principle #6Universality (Multi-functionality)

Solution Approach 2:

The patent combines multiple stabilizing mechanisms into a unified formulation system where polyphenols, vitamins, and amino acids work synergistically rather than independently. The citrate-phosphate buffer system merges pH control and ionic strength regulation. This integrated approach consolidates multiple stabilization functions into a cohesive formulation strategy.

Inventive Principle:
Principle #5Merging (Combining)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The composition effectively stabilizes phycocyanin, preventing degradation and maintaining its blue color and biological properties for at least two months, even under varying temperature and humidity conditions, and when combined with other excipients or active agents, while effectively reducing skin aging signs and promoting wound healing.

Implementation Method 1

At rest, phycocyanin has a blue colour and a red fluorescence. The pigment, respectively the chromophore, of phycocyanin is phycocyanobilin.

Methodology Applied
Scientific EffectAbsorption (EM radiation): Absorption (EM radiation)

Implementation Method 2

the chromophore is sensitive to oxidation which may also reduce the color

Methodology Applied
Scientific EffectOxidation: Oxidation

Implementation Method 3

pH adjusters in an effective amount to set the pH from 6.0 to 6.5

Methodology Applied
Scientific EffectpH regulation:

Implementation Method 4

an effective amount of a citrate phosphate buffer

Methodology Applied
Scientific EffectBuffering:

Data Source

PatentEP3760184B1Composition comprising stabilized phycocyanin and its uses
Publication Date: 2023.02.15 CALICEA

AI summary

This invention is directed to a composition comprising, in a topically acceptable medium, phycocyanin and a combination of specific stabilizers. It also pertains to a cosmetic or dermatological formulation comprising this composition in a cosmetic or dermatological acceptable medium, respectively. This invention is further directed to a cosmetic method for reducing the signs of skin aging, comprising topically applying onto skin the above composition or cosmetic formulation, and to the above composition or dermatological formulation for its use in the treatment of wounds.