Phyllanthus Niruri Low Molecular Weight Fraction Extraction
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Solution Overview
Problem
Existing skin care compositions using Phyllanthus niruri extracts are not equally effective in promoting tropoelastin and collagen formation, and most do not provide significant anti-inflammatory or skin lightening benefits, particularly due to the presence of high molecular weight species that inhibit their activity.
Innovation Solution
A composition comprising a low molecular weight fraction of Phyllanthus niruri, extracted using a water-based solvent and isolated through gel filtration to remove species above 100,000 daltons, is used in a topical formulation to enhance skin elasticity, reduce inflammation, and lighten skin tone.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If water extract of Phyllanthus niruri is used, then collagenase inhibition and tropoelastin promotion are improved, but high molecular weight species (>100,000 daltons) inhibit activity and reduce effectiveness
Solution Approach 1:
The patent applies extraction by isolating the low molecular weight fraction (<100,000 daltons) from the water extract of Phyllanthus niruri through gel filtration chromatography. This removes the harmful high molecular weight species while retaining the beneficial low molecular weight compounds that promote collagenase inhibition and tropoelastin formation.
Solution Approach 2:
The patent segments the water extract into different molecular weight fractions using gel filtration. By separating the extract into low molecular weight (<100,000 daltons) and high molecular weight (>100,000 daltons) fractions, the beneficial activities are concentrated in the low molecular weight fraction while eliminating the inhibitory effects of high molecular weight species.
2Reliability
If low molecular weight fraction is isolated through gel filtration, then activity is enhanced, but manufacturing complexity increases
Solution Approach 1:
The beneficial low molecular weight fraction is extracted and isolated from the complex water extract using gel filtration chromatography. This extraction process concentrates the active ingredients and removes inactive or inhibitory high molecular weight components, enhancing biological activity despite the added manufacturing step.
3Adaptability or versatility
If conventional Phyllanthus niruri extracts are used, then skin care benefits are provided, but anti-inflammatory and skin lightening effects are insufficient
Solution Approach 1:
The patent extracts and isolates the low molecular weight fraction that contains the specific bioactive compounds responsible for anti-inflammatory and skin lightening effects. This fractionation process enriches these beneficial compounds while removing components that do not contribute to these activities, thereby enhancing multi-functionality and reliability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The low molecular weight fraction of Phyllanthus niruri significantly inhibits collagenase, promotes tropoelastin and collagen formation, provides effective anti-inflammatory effects, and achieves noticeable skin lightening, improving skin appearance and reducing signs of aging.
Implementation Method 1
contacting Phyllanthus niruri with a solvent comprising water for a time period sufficient to form a water extract of Phyllanthus niruri
Implementation Method 2
isolating a low molecular weight fraction from said water extract of Phyllanthus niruri
Data Source
AI summary
A method of making a low molecular weight fraction of Phyllanthus niruri is provided. Such low molecular weight fraction is particularly useful for treating the skin, for example skin in need of treatment for signs of aging, for reducing inflammation, or for skin lightening.