Phytase Pre-treatment for Plant Protein Acidification Speed

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Solution Overview

Problem

The acidification process for producing plant-based fermented food products, such as yogurt alternatives, is slower than traditional dairy yogurt, leading to increased production time and risk of contamination due to prolonged neutral pH conditions.

Innovation Solution

Incorporating phytase into the process to accelerate the acidification speed by incubating a solution containing plant protein with phytase before fermentation with lactic acid bacteria, thereby reducing processing time and minimizing contamination risks.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Speed

If traditional dairy yogurt process is used for plant-based fermented food products, then the acidification process is slower, but the processing time is longer

Engineering Contradiction:
Improveacidification speedVSAvoidprocessing time
Core Design Contradiction:
SpeedVSLoss of time

Solution Approach 1:

The patent applies preliminary action by pre-incubating plant proteins with phytase before fermentation to break down phytic acid and release bound phosphates. This preparatory step modifies the substrate to make it more accessible to lactic acid bacteria, thereby accelerating the subsequent acidification process and reducing overall processing time.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent changes chemical parameters by adding phytase enzyme to modify the chemical composition of the plant protein solution. The enzyme catalyzes the hydrolysis of phytic acid, releasing inorganic phosphate and altering the pH dynamics, which creates more favorable conditions for rapid lactic acid bacteria growth and acidification.

Inventive Principle:
Principle #35Parameter changes

2Reliability

If traditional dairy yogurt process is used for plant-based fermented food products, then the acidification process is slower, but the risk of contamination is higher

Engineering Contradiction:
Improvecontamination riskVSAvoidacidification speed
Core Design Contradiction:
ReliabilityVSSpeed

Solution Approach 1:

The patent applies preliminary anti-action by using phytase to pre-treat the plant protein solution, which creates an environment less favorable for unwanted microbial growth. The enzymatic breakdown of phytic acid and resulting pH changes occur before fermentation, providing a head start in preventing contamination during the critical early stages when the environment is still near neutral pH.

Inventive Principle:
Principle #9Preliminary anti-action

3Loss of time

If phytase is added to accelerate acidification, then the processing time is reduced, but the process complexity increases

Engineering Contradiction:
Improveprocessing timeVSAvoidprocess complexity
Core Design Contradiction:
Loss of timeVSDevice complexity

Solution Approach 1:

The patent uses phytase as an intermediary substance that facilitates the acidification process without being consumed in the final product. The enzyme acts as a mediator between the plant proteins and lactic acid bacteria, preparing the substrate and creating favorable conditions for fermentation while being easily added and removed from the process.

Inventive Principle:
Principle #24Intermediary (Mediator)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The use of phytase significantly shortens the acidification time and reduces the risk of unwanted microbial growth, resulting in a more efficient and safer production process for plant-based fermented food products.

Implementation Method 1

incubating a solution comprising a plant protein with phytase

Methodology Applied
Scientific EffectEnzymatic hydrolysis: Hydrolysis

Implementation Method 2

fermenting a solution comprising a plant protein with lactic acid bacteria

Methodology Applied
Scientific EffectFermentation: Fermentation

Data Source

PatentUS20230102612A1Accelerating the acidification speed of lactic acid bacteria
Publication Date: 2023.03.30 DSM IP ASSETS BV

AI summary

The present invention describes a method for preparing a fermented food product comprising a plant protein, comprisingincubating a solution comprising a plant protein with phytase, andfermenting a solution comprising a plant protein with lactic acid bacteria.