Piglet Feed Bacterial Enzyme Synergistic Fermentation

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Solution Overview

Problem

Current animal feed phosphorus utilization efficiency is low, leading to environmental pollution and nutritional deficiencies in pigs due to the low digestibility of phytate phosphorus, which is not effectively utilized by piglets lacking phytase enzymes.

Innovation Solution

A piglet feed based on a bacterial enzyme synergistic fermentation process using Lactobacillus plantarum, Pediococcus pentosaceus, and Aspergillus niger strains combined with protease, amylase, lipase, and cellulase enzymes, applied to a fermentation substrate, significantly improving phosphorus availability and overall nutrient utilization.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Quantity of substance

If phytase is added to hydrolyze phytic acid in plant feed, then phosphorus availability is improved, but the improvement rate is limited and cannot fully resolve phosphorus utilization issues

Engineering Contradiction:
Improvephosphorus availabilityVSAvoidphosphorus utilization rate
Core Design Contradiction:
Quantity of substanceVSProductivity

Solution Approach 1:

The patent combines multiple microorganisms (Lactobacillus plantarum, Bacillus subtilis, Aspergillus oryzae) with multiple enzymes (phytase, protease, amylase, lipase, cellulase) into a composite preparation that works synergistically. This merging approach overcomes the limitation of single phytase addition by creating a multi-functional system that simultaneously performs phosphorus release and comprehensive nutrient digestion, thereby significantly improving phosphorus utilization beyond what single phytase can achieve.

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The invention uses a composite fermentation preparation containing multiple microbial strains and enzyme types rather than a single enzyme or microorganism. This composite material approach allows the system to perform multiple functions simultaneously: phytase releases phosphorus from phytate, while protease, amylase, lipase, and cellulase break down other macronutrients, creating a synergistic effect that greatly enhances overall phosphorus availability and nutrient utilization.

Inventive Principle:
Principle #40Composite materials

2Quantity of substance

If inorganic phosphorus sources are added to meet pig growth needs, then phosphorus supply is sufficient, but phosphorus excretion increases causing environmental pollution

Engineering Contradiction:
Improvephosphorus supplyVSAvoidphosphorus excretion and environmental pollution
Core Design Contradiction:
Quantity of substanceVSObject-generated harmful factors

Solution Approach 1:

The fermented feed preparation contains microorganisms and enzymes that actively work within the pig's digestive system to release phosphorus from phytate on-site. This self-service mechanism allows the feed to provide its own phosphorus through biological hydrolysis, reducing the need for additional inorganic phosphorus supplements and minimizing phosphorus waste in excreta, thereby decreasing environmental pollution.

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The patent changes the chemical state of phosphorus from bound phytate phosphorus to free phosphorus through enzymatic hydrolysis during fermentation and digestion. This parameter change in phosphorus availability allows for reduced inorganic phosphorus addition while meeting nutritional requirements, thereby reducing phosphorus excretion and environmental impact.

Inventive Principle:
Principle #35Parameter changes

3Quantity of substance

If phytate phosphorus is used in feed, then phosphorus content is high, but digestibility and utilization are low due to interaction with anti-nutrient substances

Engineering Contradiction:
Improvephosphorus contentVSAvoidnutrient digestibility
Core Design Contradiction:
Quantity of substanceVSReliability

Solution Approach 1:

The patent applies preliminary enzymatic hydrolysis through fermentation with phytase and other enzymes before the feed is consumed by pigs. This preliminary action breaks down phytate phosphorus and anti-nutrient substances in advance, converting bound phosphorus into available forms and reducing interactions that would otherwise hinder digestion, thereby improving both phosphorus availability and overall nutrient digestibility.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The fermented preparation acts as an intermediary that mediates between phytate phosphorus and the pig's digestive system. The enzymes in the preparation (phytase, protease, amylase, lipase, cellulase) serve as intermediaries that break down complex compounds into absorbable forms, facilitating phosphorus release and reducing negative interactions with anti-nutrient substances, thereby improving digestibility and utilization.

Inventive Principle:
Principle #24Intermediary (Mediator)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The bacterial enzyme synergistic fermentation feed increases phosphorus utilization by 50%, enhancing growth performance and digestibility of feed nutrients, reducing phosphorus excretion and environmental impact.

Implementation Method 1

add phytase to the feed to hydrolyze the phytic acid in the plant feed

Methodology Applied
Scientific EffectHydrolysis: Hydrolysis

Implementation Method 2

bacterial enzyme synergistic fermentation process

Methodology Applied
Scientific EffectFermentation: Fermentation

Implementation Method 3

enzyme preparation, and a bacterial strain... combined with protease, amylase, lipase, and cellulase enzymes

Methodology Applied
Scientific EffectEnzyme catalysis: Enzyme

Data Source

PatentUS11832634B2Piglet feed based on bacterial enzyme synergistic fermentation process and preparation method thereof
Publication Date: 2023.12.05 SICHUAN AGRI UNIV

AI summary

Disclosed is a piglet feed based on bacteria enzyme synergistic fermentation process. The piglet feed is composed of basic components and bacteria enzyme synergistic fermentation feed. Basic components include soybean protein concentrate, whey powder, fish meal, sodium chloride, choline chloride, stone powder, calcium hydrogen phosphate, composite vitamins, composite trace elements, and composite amino acids. The bacterial enzyme synergistic fermentation feed includes a fermentation substrate, an enzyme preparation, and a bacterial strain. The bacterial enzyme synergistic fermentation feed can not only improve the production performance of piglets, but also improve the utilization rate of feed nutrients, especially the utilization rate of feed phosphorus, thereby reducing the excretion of phosphorus in feces.