Pineapple Germplasm Breeding Under Staged Cold and Heat Domestication
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Solution Overview
Problem
The existing breeding methods for Ananas comosus (pineapple) are limited by temperature tolerance, making it difficult to cultivate in areas with temperatures below 8°C in winter and above 38°C in summer, thus restricting its planting area and productivity.
Innovation Solution
A breeding method involving controlled greenhouse conditions, specific fertilization and irrigation regimes, and temperature fluctuations to domesticate cold-resistant and high-temperature-resistant germplasm, including stages of seedling, flowering, and fruit development with targeted fertilizers and temperature adjustments.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If conventional breeding methods are used for Ananas comosus, then the plant can grow in traditional tropical climates, but the planting area is limited to regions with lowest temperature above 8°C in winter
Solution Approach 1:
The patent applies parameter changes by systematically adjusting temperature parameters during different growth stages. The method involves gradual temperature reduction from 28-30°C during seedling stage to 4-6°C during cold resistance training, then progressive warming to 36-38°C during flowering and fruiting stages. This controlled parameter variation enables the plant to acclimate to extreme temperatures while maintaining physiological functions, thereby expanding the suitable planting area to regions with lowest temperatures above 4°C in winter.
2Reliability
If the plant is exposed to extreme temperatures to expand planting area, then temperature tolerance is improved, but the plant may suffer from thermal stress damage
Solution Approach 1:
The patent implements preliminary action through progressive cold resistance training during the seedling stage. The temperature is gradually reduced from 28-30°C to 4-6°C over multiple days, allowing the plant to acclimate and develop cold tolerance mechanisms before being exposed to full cold stress. This staged approach primes the plant's physiological systems, enabling it to withstand subsequent extreme temperature conditions without suffering severe thermal stress damage.
Solution Approach 2:
The patent applies dynamics by implementing flexible temperature adjustment protocols that respond to plant developmental stages and environmental conditions. The temperature schedule is not fixed but dynamically adjusted based on growth phase requirements - cooler temperatures (28-30°C) during seedling stage, extreme cold exposure (4-6°C) during cold resistance training, and warm temperatures (36-38°C) during flowering and fruiting. This dynamic temperature management enables the plant to tolerate extreme temperatures while minimizing thermal stress damage through appropriate timing and progression.
3Reliability
If trace elements are added to improve resistance, then the plant's resistance to low-temperature and high-temperature damages is improved, but the fertilization process becomes more complex
Solution Approach 1:
The patent applies merging by integrating multiple trace elements (Mg, B, Zn, Fe, Mn, Mo) into a unified foliar fertilizer application system. Rather than applying each element separately through complex individual treatments, the method combines these essential elements in a single foliar spray solution that is applied at specific stages (7 days after first root irrigation and fertilization, and again 15 days after first foliar fertilization). This merged approach simplifies the fertilization process while maintaining comprehensive nutrient supply for improved temperature resistance.
Solution Approach 2:
The patent uses foliar fertilizer as an intermediary medium to deliver trace elements to the plant. The foliar fertilizer solution acts as a carrier that efficiently transports multiple trace elements directly to the leaf surfaces, where they are absorbed and utilized for enhancing temperature resistance. This intermediary approach simplifies element delivery compared to soil application methods, as it bypasses complex soil chemistry interactions and provides direct access to the plant's photosynthetic and metabolic systems.
Data Source
AI summary
Disclosed is a breeding method for cultivating a cold-resistant and high-temperature-resistant Ananas comosus germplasm. The breeding method mainly comprises the following technical key points: creating a controllable greenhouse environment which simulates a natural environment for production, performing low-temperature domestication culture in two stages of seedling planting stage and breeding stage in a growth period, and performing high-temperature domestication culture in multiple stages of seedling growth stage, flowering stage, fruit stage and breeding stage, and for each key stage according to a growth pattern of Ananas comosus, providing precise technical implementation key points on time point control and management measure digitalization, so as to facilitate accurate implementation of operations. The method is simple and convenient for large-scale breeding production, multiple rounds of seedlings can be harvested in one round of production, and the seedling production efficiency of the cold-resistant and high-temperature-resistant Ananas comosus germplasm is multiplied.