Pinkpepper Hemp DNA Primer Sets for Legal High-CBD Identification
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Solution Overview
Problem
Current South Korean laws classify all Cannabis sativa L. products as narcotics, hindering the development and use of medical hemp cultivars with high CBD content, despite international recognition of its therapeutic benefits, and traditional domestic hemp cultivars have low CBD levels unsuitable for medical and industrial purposes.
Innovation Solution
Development of a new Cannabis sativa L. cultivar named Pinkpepper, achieved through artificial crossing of KHV2 and India genetic resources, with high CBDA and CBD content, suitable for smart farm cultivation, allowing two harvests per year, and resistant to flowering daylight length, along with a primer set for identification.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If traditional domestic hemp cultivars are used, then cultivation is legally permissible under current South Korean laws, but CBD content is low and unsuitable for medical purposes
Solution Approach 1:
The patent changes the genetic parameters of hemp cultivars through artificial crossing between KHV2 and India genetic resources, creating Pinkpepper with high CBD content (≥1.0% dry weight) while maintaining THC content below the legal threshold of 0.3%, thus resolving the contradiction between CBD quantity and legal reliability
Solution Approach 2:
The patent creates a composite genetic structure by crossing two distinct genetic resources (KHV2 and India), combining their advantageous traits to produce a hybrid cultivar that simultaneously achieves high CBD content and legal compliance
2Quantity of substance
If hemp cultivars with high CBD content are developed, then medical utility is enhanced, but identification and verification become more difficult
Solution Approach 1:
The patent establishes a feedback mechanism for cultivar identification by developing specific primer sets that amplify and detect unique DNA markers of Pinkpepper, enabling verification of high CBD content and authenticating the cultivar identity through molecular biology techniques
Solution Approach 2:
The patent replaces traditional phenotypic identification methods with molecular biology-based DNA marker detection, substituting mechanical/visual assessment with biochemical analysis that provides more accurate and reliable cultivar identification
3Productivity
If autoflowering cultivars are used, then productivity increases with two harvests per year, but resistance to flowering daylight length conditions varies
Solution Approach 1:
The patent introduces dynamic adaptability to the autoflowering cultivar by incorporating genetic resistance to flowering daylight length conditions, allowing the plant to maintain its autonomous flowering mechanism while adapting to varying photoperiod conditions across different cultivation environments
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Pinkpepper cultivar exhibits enhanced CBD content, suitable for medical and industrial applications, enabling efficient cultivation and identification, addressing legal and cultivation challenges.
Implementation Method 1
a primer set composition for identifying the same
Data Source
AI summary
A composition for identifying a hemp cultivar Pinkpepper or a progeny plant thereof includes the oligonucleotide primer set of SEQ ID NOs: 7 and 8, and/or the oligonucleotide primer set of SEQ ID NOs: 9 and 10. A method for identifying hemp cultivar Pinkpepper or a progeny plant thereof includes extracting genomic DNA from a hemp plant, amplifying a target sequence by having the extracted genomic DNA as template and carrying out an amplification reaction using the oligonucleotide primer set, and detecting a product produced by the application of the target sequence.