Pipette Tip Straight Pipe Section for Cell Breakage Control

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Solution Overview

Problem

Conventional pipette tips do not adequately address cell breakage and irregularity in diameter during pipetting, which affects cell aggregation and dispersion, leading to inefficient cell processing.

Innovation Solution

A pipette tip with a straight pipe section having an inner diameter of 1.0 mm to 1.5 mm and a length of 5 mm to 15 mm, designed to minimize diameter irregularity and facilitate effective cell suspension handling through controlled sucking and discharging.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Manufacturing precision

If conventional pipette tips with varied diameters are used, then liquid transport function is achieved, but cell breakage control and diameter irregularity increase

Engineering Contradiction:
Improvediameter irregularityVSAvoidcell breakage control
Core Design Contradiction:
Manufacturing precisionVSEase of operation

Solution Approach 1:

The patent applies parameter changes by specifying precise dimensional parameters for the straight pipe section: inner diameter of 1.0-1.5mm and length of 5-15mm. This quantitative parameter optimization resolves the contradiction by controlling diameter irregularity while maintaining effective cell breakage functionality through the defined geometric parameters.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent applies local quality by creating a straight pipe section with uniform diameter at the leading end, distinct from other sections of the pipette tip. This localized uniformity in the straight pipe section specifically addresses diameter irregularity while the overall tip structure maintains its liquid transport capabilities, thus resolving the contradiction between precision and operational effectiveness.

Inventive Principle:
Principle #3Local quality

2Manufacturing precision

If pipette tip with straight pipe section is used, then cell aggregation control is improved, but device complexity increases

Engineering Contradiction:
Improvecell aggregation uniformityVSAvoidtip structure
Core Design Contradiction:
Manufacturing precisionVSDevice complexity

Solution Approach 1:

The patent applies segmentation by dividing the pipette tip into distinct functional sections: a straight pipe section at the leading end and a main body section. This segmentation allows the straight pipe section to specifically control cell aggregation uniformity while the main body maintains standard pipette tip functionality, thus improving precision without excessive complexity increase.

Inventive Principle:
Principle #1Segmentation

Data Source

PatentEP3272851B1Pipette tip and pipetting method
Publication Date: 2020.03.04 PANASONIC HOLDINGS CORP
  • EP3272851B1 patent drawingFigure 1A
  • EP3272851B1 patent drawingFigure 1B
  • EP3272851B1 patent drawingFigure 2(a)~2(c)

AI summary

A pipette tip includes a main body section; and a straight pipe section disposed at the leading end of the main body section, in which the inner diameter of the straight pipe section is 0.8 mm to 1.5 mm inclusive, and the length of the straight pipe section is 5 mm to 15 mm inclusive.