Pipetting Tip Alignment Scanning for Multi-Tip Position Verification
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Solution Overview
Problem
Existing contactless measurement systems for pipetting tips in automated pipetting systems are limited to measuring one tip at a time and do not effectively calculate and confirm the correct positioning of an array of pipetting tips, which can lead to misalignment and disruption in high-throughput laboratory protocols.
Innovation Solution
A method and system using an image capture device with a sensor to map and calculate the alignment of multiple pipetting tips relative to a virtual grid, incorporating a data processor to determine center positions and provide alignment information, allowing for contactless measurement of an array of pipetting tips.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If an image capture device is used to measure the position of pipetting tips, then measurement precision is improved, but the ability to measure multiple tips simultaneously is limited
Solution Approach 1:
The patent divides the measurement task into two stages: first capturing a wide-field overview image to identify all tip positions, then performing detailed measurements on each tip. This segmentation allows the system to efficiently handle multiple tips while maintaining measurement precision through the two-stage approach.
Solution Approach 2:
The patent transitions from measuring a single tip in isolation to capturing an array of tips in a two-dimensional grid layout. By utilizing the spatial arrangement of multiple tips in the field of view, the system measures multiple tips simultaneously without sacrificing precision, effectively adding the dimension of parallel measurement capability.
2Object-affected harmful factors
If contactless measurement is implemented, then contamination risk is reduced, but the ability to detect and confirm correct positioning of multiple tips is insufficient
Solution Approach 1:
The patent implements a feedback mechanism where the image capture device continuously monitors tip positions, and the system automatically confirms correct positioning before allowing the pipetting protocol to proceed. This feedback loop ensures reliable positioning confirmation without requiring physical contact, maintaining the contactless advantage while improving reliability.
Solution Approach 2:
The system performs preliminary measurement and confirmation of tip positioning before the actual pipetting protocol begins. By validating all tip positions in advance, the system ensures reliability is established beforehand, preventing protocol disruptions and eliminating the need for contact-based verification during the procedure.
3Productivity
If multiple pipetting tips are mounted in close proximity, then productivity is improved, but alignment precision deteriorates
Solution Approach 1:
The patent applies different measurement approaches to different regions of the pipetting head. Tips that are clearly visible and properly aligned are confirmed quickly, while tips showing potential misalignment receive more detailed examination. This local quality approach allows the system to maintain high productivity for well-aligned tips while ensuring precision for potentially problematic ones.
Solution Approach 2:
The system dynamically adjusts its measurement strategy based on the observed alignment quality of each tip. If initial measurements indicate proper alignment, the system proceeds quickly; if misalignment is detected, the system automatically triggers corrective actions. This dynamic response allows the system to maintain both high productivity and precision adaptively.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Ensures fast and reliable alignment measurements of pipetting tips, reducing the risk of protocol disruption and material waste by detecting misalignments before the pipetting process, enhancing safety and efficiency in automated pipetting systems.
Implementation Method 1
Contactless measurement systems use a range of physical properties to sense the environment, e.g., light
Data Source
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Figure 5~6F
AI summary
Method of measuring the alignment of one or more pipetting tips (2) in an automated pipetting system (1) whereas the one or more pipetting tips (2) are mounted on one or more adapters (3) of a pipetting head (4), approximately perpendicular to the pipetting head (4) mounting surface, the orifices (21) at the protruding end of the one or more pipetting tips (2) are mapped with an image capture device (5) comprising a sensor (6) placed in face and distant of the orifices (21). The method further comprises sending the acquired data from mapping the orifices (21) of the one or more pipetting tips (2) to the data processor (7), generating an image of the orifices (21) of the one or more pipetting tips (2), determining the center positions of orifices (21), and delivering alignment information for the one or more pipetting tips (2). A computer program comprising instructions which, when the program is executed by a data processor (7), cause an automated pipetting system (1) to perform the method of measuring the alignment of one or more pipetting tips (2) in an automated pipetting system (1), and a contactless measurement system (10) for measuring the alignment of one or more pipetting tips (2) in an automated pipetting system (1) comprising one or more adapters (3) on a pipetting head (4) connected to a robotic pipetting arm (11) for mounting the one or more pipetting tips (2), an image capture device (5) comprising a sensor (6) joined to a worktable (9) for capturing an image of the orifices (21) of the one or more pipetting tips (2) or of the one or more adapters (3) of the pipetting head (4), a data processor (7) for receiving the image data acquired by the sensor (6) and for calculating alignment information for the one or more pipetting tips (2) with respect to a virtual regular grid assumed to the pipetting head (4) mounting surface. Use of the contactless measurement system (10) and automated pipetting system (1) comprising the contactless measurement system (10) according to the invention.